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Review
. 2015 Aug;3(4).
doi: 10.1128/microbiolspec.MDNA3-0039-2014.

The IS200/IS605 Family and "Peel and Paste" Single-strand Transposition Mechanism

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Free article
Review

The IS200/IS605 Family and "Peel and Paste" Single-strand Transposition Mechanism

S He et al. Microbiol Spectr. 2015 Aug.
Free article

Abstract

This chapter presents an analysis of the organization and distribution of the IS200/IS605 family of insertion sequences (IS). Members of this family are widespread in both bacteria and archaea. They are unusual because they use obligatory single-strand DNA intermediates, which distinguishes them from classical IS. We summarize studies of the experimental model systems IS608 (from Helicobacter pylori) and ISDra2 (from Deinococcus radiodurans) and present biochemical, genetic, and structural data that describe their transposition pathway and the way in which their transposase (an HuH rather than a DDE enzyme) catalyzes this process. The transposition of IS200/IS605 family members can be described as a "Peel-and-Paste" mechanism. We also address the probable domestication of IS200/IS605 family transposases as enzymes involved in multiplication of repeated extragenic palindromes and as potential homing endonucleases in intron-IS chimeras.

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