Prevalence of Human T-Lymphotropic Virus Types I and II in Patients With Hematological Disorders in Isfahan, Iran

doi:10.5812/jjm.8(5)2015.17201

. 2015 Jun 25;8(6):e17201.

doi: 10.5812/jjm.8(5)2015.17201. eCollection 2015 Jun.

Prevalence of Human T-Lymphotropic Virus Types I and II in Patients With Hematological Disorders in Isfahan, Iran

Mohammadreza Mahzounieh¹, Mohammadreza Ghorani², Ali Karimi³, Batoul Pourgheysari⁴, Razieh Nikoozad⁵

Affiliations

¹ Pathobiology Department, Research Institute of Zoonotic Diseases, Shahrekord University, Shahrekord, IR Iran.
² Department of Microbiology, Tehran University, Tehran, IR Iran.
³ Microbiology and Immunology Department, Shahrekord University of Medical Sciences, Shahrekord, IR Iran.
⁴ Pathology and Hematology Department, Shahrekord University of Medical Sciences, Shahrekord, IR Iran.
⁵ Department of Microbiology, Islamic Azad University, Falavarjan Branch, Falavarjan, IR Iran.

PMID: 26301057
PMCID: PMC4539791
DOI: 10.5812/jjm.8(5)2015.17201

Prevalence of Human T-Lymphotropic Virus Types I and II in Patients With Hematological Disorders in Isfahan, Iran

Mohammadreza Mahzounieh et al. Jundishapur J Microbiol. 2015.

. 2015 Jun 25;8(6):e17201.

doi: 10.5812/jjm.8(5)2015.17201. eCollection 2015 Jun.

Authors

Mohammadreza Mahzounieh¹, Mohammadreza Ghorani², Ali Karimi³, Batoul Pourgheysari⁴, Razieh Nikoozad⁵

Affiliations

¹ Pathobiology Department, Research Institute of Zoonotic Diseases, Shahrekord University, Shahrekord, IR Iran.
² Department of Microbiology, Tehran University, Tehran, IR Iran.
³ Microbiology and Immunology Department, Shahrekord University of Medical Sciences, Shahrekord, IR Iran.
⁴ Pathology and Hematology Department, Shahrekord University of Medical Sciences, Shahrekord, IR Iran.
⁵ Department of Microbiology, Islamic Azad University, Falavarjan Branch, Falavarjan, IR Iran.

PMID: 26301057
PMCID: PMC4539791
DOI: 10.5812/jjm.8(5)2015.17201

Abstract

Background: Human T-cell lymphotropic virus types Ι and ΙΙ (HTLV-Ι and HTLV-II) are deltaretroviruses which may cause leukemia, lymphoma and HTLV-associated myelopathy/tropical spastic paraparesis (HAM/TSP). In addition, HTLV-1 may be related to thalassemia and hemophilia cases after blood transfusion.

Objectives: The aim of this study was evaluation of the prevalence of HTLVs in patients with hematological disorders (leukemia, thalassemia, lymphoma and hemophilia).

Patients and methods: This cross-sectional study was conducted during April to October 2012. A total of 101 serum samples were collected from patients and were stored at -20ºC. DNA was extracted from serum by an extraction kit. The extracted DNA was tested by polymerase chain reaction (PCR) for detection of HTLV-Ι and HTLV-II pol and tax gene sequences, respectively. Samples were collected from 67 (66.33%), 20 (19.80%), 4 (3.96%), and 10 (9.90%) patients with thalassemia, leukemia, lymphoma and hemophilia, respectively.

Results: One thalassemia sample was HTLV-Ι positive, but none of the samples contained the genome of HTLV-II. The prevalence of HTLV-Ι in this study in patients with hematological disorders was 0.99%.

Conclusions: The prevalence of HTLV-Ι in hematological disorders was similar to that of other parts of Iran. The present study revealed that HTLV-Ι screening should be performed before blood transfusion to reduce the risk of virus transmission in patients with hematological disorders. More study should be performed to detect these viruses in blood donors.

Keywords: Hematological Diseases; Human T-lymphotropic Virus; Iran.

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Figures

**Figure 1.. Amplification of the pol gene in Patients with Thalassemia**
A 668 bp fragment was amplified from the pol region of HTLV-Ι genome. Lane 1, 100 bp marker; lane 2, positive control (TL-Oml); lane 3, negative sample; lane 4, positive sample; lane 5, negative control.

See this image and copyright information in PMC

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References

1. Poiesz BJ, Ruscetti FW, Gazdar AF, Bunn PA, Minna JD, Gallo RC. Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T-cell lymphoma. Proc Natl Acad Sci U S A. 1980;77(12):7415–9. - PMC - PubMed
1. Kalyanaraman VS, Sarngadharan MG, Poiesz B, Ruscetti FW, Gallo RC. Immunological properties of a type C retrovirus isolated from cultured human T-lymphoma cells and comparison to other mammalian retroviruses. J Virol. 1981;38(3):906–15. - PMC - PubMed
1. Matsuoka M, Jeang KT. Human T-cell leukaemia virus type 1 (HTLV-1) infectivity and cellular transformation. Nat Rev Cancer. 2007;7(4):270–80. doi: 10.1038/nrc2111. - DOI - PubMed
1. Johnson JM, Harrod R, Franchini G. Molecular biology and pathogenesis of the human T-cell leukaemia/lymphotropic virus Type-1 (HTLV-1). Int J Exp Pathol. 2001;82(3):135–47. - PMC - PubMed
1. Monplaisir N, Neisson-Vernant C, Bouillot M, Duc-Dodon M, Ugarte E, Valette I, et al. HTLV-I maternal transmission in Martinique, using serology and polymerase chain reaction. AIDS Res Hum Retroviruses. 1993;9(9):869–74. - PubMed

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[1] Poiesz BJ, Ruscetti FW, Gazdar AF, Bunn PA, Minna JD, Gallo RC. Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T-cell lymphoma. Proc Natl Acad Sci U S A. 1980;77(12):7415–9. - PMC - PubMed

[2] Poiesz BJ, Ruscetti FW, Gazdar AF, Bunn PA, Minna JD, Gallo RC. Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T-cell lymphoma. Proc Natl Acad Sci U S A. 1980;77(12):7415–9. - PMC - PubMed

[3] Kalyanaraman VS, Sarngadharan MG, Poiesz B, Ruscetti FW, Gallo RC. Immunological properties of a type C retrovirus isolated from cultured human T-lymphoma cells and comparison to other mammalian retroviruses. J Virol. 1981;38(3):906–15. - PMC - PubMed

[4] Kalyanaraman VS, Sarngadharan MG, Poiesz B, Ruscetti FW, Gallo RC. Immunological properties of a type C retrovirus isolated from cultured human T-lymphoma cells and comparison to other mammalian retroviruses. J Virol. 1981;38(3):906–15. - PMC - PubMed

[5] Matsuoka M, Jeang KT. Human T-cell leukaemia virus type 1 (HTLV-1) infectivity and cellular transformation. Nat Rev Cancer. 2007;7(4):270–80. doi: 10.1038/nrc2111. - DOI - PubMed

[6] Matsuoka M, Jeang KT. Human T-cell leukaemia virus type 1 (HTLV-1) infectivity and cellular transformation. Nat Rev Cancer. 2007;7(4):270–80. doi: 10.1038/nrc2111. - DOI - PubMed

[7] Johnson JM, Harrod R, Franchini G. Molecular biology and pathogenesis of the human T-cell leukaemia/lymphotropic virus Type-1 (HTLV-1). Int J Exp Pathol. 2001;82(3):135–47. - PMC - PubMed

[8] Johnson JM, Harrod R, Franchini G. Molecular biology and pathogenesis of the human T-cell leukaemia/lymphotropic virus Type-1 (HTLV-1). Int J Exp Pathol. 2001;82(3):135–47. - PMC - PubMed

[9] Monplaisir N, Neisson-Vernant C, Bouillot M, Duc-Dodon M, Ugarte E, Valette I, et al. HTLV-I maternal transmission in Martinique, using serology and polymerase chain reaction. AIDS Res Hum Retroviruses. 1993;9(9):869–74. - PubMed

[10] Monplaisir N, Neisson-Vernant C, Bouillot M, Duc-Dodon M, Ugarte E, Valette I, et al. HTLV-I maternal transmission in Martinique, using serology and polymerase chain reaction. AIDS Res Hum Retroviruses. 1993;9(9):869–74. - PubMed

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Prevalence of Human T-Lymphotropic Virus Types I and II in Patients With Hematological Disorders in Isfahan, Iran

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Prevalence of Human T-Lymphotropic Virus Types I and II in Patients With Hematological Disorders in Isfahan, Iran

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