Defining the structural characteristics of annexin V binding to a mimetic apoptotic membrane

doi:10.1007/s00249-015-1068-z

. 2015 Dec;44(8):697-708.

doi: 10.1007/s00249-015-1068-z. Epub 2015 Aug 14.

Defining the structural characteristics of annexin V binding to a mimetic apoptotic membrane

Jingxiong Lu^{1

2}, Anton P Le Brun³, Seong Hoong Chow¹, Takuya Shiota¹, Bo Wang¹, Tsung-Wu Lin⁴, Guei-Sheung Liu⁵, Hsin-Hui Shen^{6

7}

Affiliations

¹ School of Biomedical Sciences, Monash University, Melbourne, 3800, Australia.
² Faculty of Engineering, Monash University, Melbourne, 3800, Australia.
³ The Bragg Institute, Australian Nuclear Science and Technology Organization (ANSTO), New Illawarra Rd, Lucas Heights, NSW, 2234, Australia.
⁴ Department of Chemistry, Tunghai University, No. 181, Sec. 3, Taichung Port Rd., Taichung, 40704, Taiwan.
⁵ Centre for Eye Research Australia, Department of Ophthalmology, University of Melbourne, East Melbourne, Australia.
⁶ School of Biomedical Sciences, Monash University, Melbourne, 3800, Australia. hsin-hui.shen@monash.edu.
⁷ Faculty of Engineering, Monash University, Melbourne, 3800, Australia. hsin-hui.shen@monash.edu.

PMID: 26271933
DOI: 10.1007/s00249-015-1068-z

Defining the structural characteristics of annexin V binding to a mimetic apoptotic membrane

Jingxiong Lu et al. Eur Biophys J. 2015 Dec.

. 2015 Dec;44(8):697-708.

doi: 10.1007/s00249-015-1068-z. Epub 2015 Aug 14.

Authors

Jingxiong Lu^{1

2}, Anton P Le Brun³, Seong Hoong Chow¹, Takuya Shiota¹, Bo Wang¹, Tsung-Wu Lin⁴, Guei-Sheung Liu⁵, Hsin-Hui Shen^{6

7}

Affiliations

¹ School of Biomedical Sciences, Monash University, Melbourne, 3800, Australia.
² Faculty of Engineering, Monash University, Melbourne, 3800, Australia.
³ The Bragg Institute, Australian Nuclear Science and Technology Organization (ANSTO), New Illawarra Rd, Lucas Heights, NSW, 2234, Australia.
⁴ Department of Chemistry, Tunghai University, No. 181, Sec. 3, Taichung Port Rd., Taichung, 40704, Taiwan.
⁵ Centre for Eye Research Australia, Department of Ophthalmology, University of Melbourne, East Melbourne, Australia.
⁶ School of Biomedical Sciences, Monash University, Melbourne, 3800, Australia. hsin-hui.shen@monash.edu.
⁷ Faculty of Engineering, Monash University, Melbourne, 3800, Australia. hsin-hui.shen@monash.edu.

PMID: 26271933
DOI: 10.1007/s00249-015-1068-z

Abstract

Annexin V is of crucial importance for detection of the phosphatidylserine of apoptotic cell membranes. However, the manner in which different amounts of phosphatidylserine at the membrane surface at different stages of apoptosis contribute to binding of annexin V is unclear. We have used a quartz crystal microbalance combined with dissipative monitoring (QCM-D) and neutron reflectivity to characterize binding of human annexin V to supported bilayers of different phospholipid composition. We created model apoptotic bilayers of 1-palmitoyl-2-oleoyl-sn-glycerophosphocholine and 1-palmitoyl-2-oleoyl-sn-glycerophosphoserine (POPS) in the ratios 19:1, 9:1, 6.7:1, 4:1, 3:1, and 2:1 (w/w) in the presence of 2.5 mM CaCl2. QCM-D data revealed that annexin V bound less to supported fluid lipid bilayers with higher POPS content (>25 % POPS). Neutron reflectivity was used to further characterize the detailed composition of lipid bilayers with membrane-bound annexin V. Analysis confirmed less annexin V binding with higher POPS content, that bound annexin V formed a discrete layer above the lipid bilayer with little effect on the overall structure of the membrane, and that the thickness and volume fraction of the annexin V layer varied with POPS content. From these results we show that the POPS content of the outer surface of lipid bilayers affects the structure of membrane-bound annexin V.

Keywords: Annexin V; Apoptosis and lipid bilayer; Neutron reflectivity; Solid–liquid interface.

PubMed Disclaimer

Cited by

Molecular Sensing and Manipulation of Protein Oligomerization in Membrane Nanotubes with Bolaamphiphilic Foldamers.
Aftahy K, Arrasate P, Bashkirov PV, Kuzmin PI, Maurizot V, Huc I, Frolov VA. Aftahy K, et al. J Am Chem Soc. 2023 Nov 22;145(46):25150-25159. doi: 10.1021/jacs.3c05753. Epub 2023 Nov 10. J Am Chem Soc. 2023. PMID: 37948300 Free PMC article.
A Quenched Annexin V-Fluorophore for the Real-Time Fluorescence Imaging of Apoptotic Processes In Vitro and In Vivo.
Kim H, Kim HY, Lee EY, Choi BK, Jang H, Choi Y. Kim H, et al. Adv Sci (Weinh). 2020 Oct 28;7(24):2002988. doi: 10.1002/advs.202002988. eCollection 2020 Dec. Adv Sci (Weinh). 2020. PMID: 33344139 Free PMC article.

References

1. J Nucl Med. 2008 Jun;49 Suppl 2:81S-95S - PubMed
1. J Biol Chem. 1990 Oct 15;265(29):17420-3 - PubMed
1. Nat Rev Mol Cell Biol. 2005 Jun;6(6):449-61 - PubMed
1. Nat Immunol. 2011 May;12(5):373-5 - PubMed
1. Biophys J. 1998 Aug;75(2):734-44 - PubMed

Publication types

Actions

MeSH terms

Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions

Substances

Actions
Actions

LinkOut - more resources

Full Text Sources
- Springer

[1] J Nucl Med. 2008 Jun;49 Suppl 2:81S-95S - PubMed

[2] J Nucl Med. 2008 Jun;49 Suppl 2:81S-95S - PubMed

[3] J Biol Chem. 1990 Oct 15;265(29):17420-3 - PubMed

[4] J Biol Chem. 1990 Oct 15;265(29):17420-3 - PubMed

[5] Nat Rev Mol Cell Biol. 2005 Jun;6(6):449-61 - PubMed

[6] Nat Rev Mol Cell Biol. 2005 Jun;6(6):449-61 - PubMed

[7] Nat Immunol. 2011 May;12(5):373-5 - PubMed

[8] Nat Immunol. 2011 May;12(5):373-5 - PubMed

[9] Biophys J. 1998 Aug;75(2):734-44 - PubMed

[10] Biophys J. 1998 Aug;75(2):734-44 - PubMed

Save citation to file

Email citation

Add to Collections

Add to My Bibliography

Your saved search

Create a file for external citation management software

Your RSS Feed

Defining the structural characteristics of annexin V binding to a mimetic apoptotic membrane

Affiliations

Defining the structural characteristics of annexin V binding to a mimetic apoptotic membrane

Authors

Affiliations

Abstract

Similar articles

Cited by

References

Publication types

MeSH terms

Substances

LinkOut - more resources

Full Text Sources