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. 2015 Nov;114(11):4251-7.
doi: 10.1007/s00436-015-4663-0. Epub 2015 Aug 14.

Schistosoma japonicum protein SjP40 inhibits TGF-β1-induced activation of hepatic stellate cells

Xiaolei Sun  1 , Lingbo Zhang  1 , Jianxin Wang  1   2 , Jinling Chen  1 , Dandan Zhu  1 , Pei Shen  1   2   3 , Xue He  1   4 , Jing Pan  1 , Wenxia Peng  1 , Yinong Duan  5
Affiliations

Schistosoma japonicum protein SjP40 inhibits TGF-β1-induced activation of hepatic stellate cells

Xiaolei Sun et al. Parasitol Res. 2015 Nov.

Abstract

SjP40 is a major egg antigen of Schistosoma japonicum. In the present study, the authors investigated the effect of SjP40 in vitro on transforming growth factor-β1 (TGF-β1)- stimulated hepatic stellate cells (HSCs). LX-2, an immortalized human HSC line, was treated with purified recombinant SjP40 (rSjP40) in the presence or absence of TGF-β1. Quantitative real-time polymerase chain reaction and western blot analysis were performed to determine messenger ribonucleic acid and protein of fibrogenic genes and TGF-β signaling pathway. The results showed that expression of fibrogenic genes was significantly reduced by rSjP40. Furthermore, rSjP40 also suppressed the TGF-β1-induced upregulation of Smads and ERK proteins. We also found that the effect of rSjP40 on HSCs was similar to SB431542, an inhibitor of type I TGF-β receptor. In conclusion, the data suggest that SjP40 attenuates HSC activation, which might be, at least in part, mediated by inhibiting the TGF-β and ERK signaling pathways.

Keywords: ERK; Hepatic fibrosis; Hepatic stellate cells; P40; Schistosoma japonicum; TGF-β1.

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