Spermatogonial stem cells specific marker identification in channel catfish, Ictalurus punctatus and blue catfish, I. furcatus

doi:10.1007/s10695-015-0106-1

. 2015 Dec;41(6):1545-56.

doi: 10.1007/s10695-015-0106-1. Epub 2015 Aug 7.

Spermatogonial stem cells specific marker identification in channel catfish, Ictalurus punctatus and blue catfish, I. furcatus

Mei Shang^{1

2}, Baofeng Su^{3

4}, Elizabeth A Lipke⁵, Dayan A Perera^{3

6}, Chao Li^{3

7}, Zhenkui Qin³, Yun Li³, David A Dunn^{5

8}, Sehriban Cek^{3

9}, Eric Peatman³, Rex A Dunham¹⁰

Affiliations

¹ School of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, Auburn, AL, 36849, USA. mzs0040@tigermail.auburn.edu.
² Key Laboratory of Freshwater Aquatic Biotechnology and Genetic Breeding, Heilongjiang Fisheries Research Institute, Chinese Academy of Fishery Sciences, Ministry of Agriculture, Harbin, 150070, China. mzs0040@tigermail.auburn.edu.
³ School of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, Auburn, AL, 36849, USA.
⁴ Key Laboratory of Freshwater Aquatic Biotechnology and Genetic Breeding, Heilongjiang Fisheries Research Institute, Chinese Academy of Fishery Sciences, Ministry of Agriculture, Harbin, 150070, China.
⁵ Department of Chemical Engineering, Auburn University, Auburn, AL, 36849, USA.
⁶ Gus R. Douglass Land-Grant Institute, West Virginia State University, Institute, WV, 25112, USA.
⁷ Marine Science and Engineering College, Qingdao Agricultural University, Qingdao, 266109, China.
⁸ Department of Biological Sciences, State University of New York at Oswego, Oswego, NY, 13126-3599, USA.
⁹ Faculty of Marine Science and Technology, Mustafa Kemal University, 31200, İskenderun, Hatay, Turkey.
¹⁰ School of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, Auburn, AL, 36849, USA. dunhara@auburn.edu.

PMID: 26251285
DOI: 10.1007/s10695-015-0106-1

Spermatogonial stem cells specific marker identification in channel catfish, Ictalurus punctatus and blue catfish, I. furcatus

Mei Shang et al. Fish Physiol Biochem. 2015 Dec.

. 2015 Dec;41(6):1545-56.

doi: 10.1007/s10695-015-0106-1. Epub 2015 Aug 7.

Authors

Mei Shang^{1

2}, Baofeng Su^{3

4}, Elizabeth A Lipke⁵, Dayan A Perera^{3

6}, Chao Li^{3

7}, Zhenkui Qin³, Yun Li³, David A Dunn^{5

8}, Sehriban Cek^{3

9}, Eric Peatman³, Rex A Dunham¹⁰

Affiliations

¹ School of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, Auburn, AL, 36849, USA. mzs0040@tigermail.auburn.edu.
² Key Laboratory of Freshwater Aquatic Biotechnology and Genetic Breeding, Heilongjiang Fisheries Research Institute, Chinese Academy of Fishery Sciences, Ministry of Agriculture, Harbin, 150070, China. mzs0040@tigermail.auburn.edu.
³ School of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, Auburn, AL, 36849, USA.
⁴ Key Laboratory of Freshwater Aquatic Biotechnology and Genetic Breeding, Heilongjiang Fisheries Research Institute, Chinese Academy of Fishery Sciences, Ministry of Agriculture, Harbin, 150070, China.
⁵ Department of Chemical Engineering, Auburn University, Auburn, AL, 36849, USA.
⁶ Gus R. Douglass Land-Grant Institute, West Virginia State University, Institute, WV, 25112, USA.
⁷ Marine Science and Engineering College, Qingdao Agricultural University, Qingdao, 266109, China.
⁸ Department of Biological Sciences, State University of New York at Oswego, Oswego, NY, 13126-3599, USA.
⁹ Faculty of Marine Science and Technology, Mustafa Kemal University, 31200, İskenderun, Hatay, Turkey.
¹⁰ School of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, Auburn, AL, 36849, USA. dunhara@auburn.edu.

PMID: 26251285
DOI: 10.1007/s10695-015-0106-1

Abstract

Testicular germ cells of channel catfish, Ictalurus punctatus, and blue catfish, I. furcatus were separated into four layers with Percoll density gradient centrifugation, containing different cell types (40% in the first layer were spermatogonial stem cells, SSCs). Expression of seventeen genes was analyzed for cells from different layers by real-time quantitative PCR. Pfkfb4, Urod, Plzf, Integrin6, IntegrinV, Thy1 and Cdh1 genes showed the same expression change pattern in both channel and blue catfish as these genes were down-regulated in the spermatocytes and even more so in spermatids. Plzf and Integrin6 had especially high expression in SSCs and can be used as SSCs specific markers. Sox2 gene was up-regulated in spermatocytes and even more highly up-regulated in spermatids, which indicated it could be a spermatid marker. In contrast to channel catfish, Id4, Smad5 and Prdm14 gene expressions were strongly down-regulated in spermatocyte cells, but up-regulated in spermatid cells in blue catfish. Smad5 gene was down-regulated in spermatocytes, but up-regulated in both spermatogonia and spermatids, allowing identification as a marker for spermatocytes in blue catfish. Oct4, Id4, Gfrα2, Pum2 and Prdm14 genes showed different expression patterns in the testicular germ cells of channel and blue catfish. This may be a partial explanation to the differing responses of channel catfish and blue catfish to induced spawning technologies. The SSCs specific markers can be used for further SSCs labeling, which can increase the SSCs sorting efficiency and be applied in various studies involving SSCs and other germ cells.

Keywords: Catfish; Density gradient centrifugation; Gene expression; Marker gene; Spermatogonia stem cell.

PubMed Disclaimer

Cited by

Models and Molecular Markers of Spermatogonial Stem Cells in Vertebrates: To Find Models in Nonmammals.
Song H, Park HJ, Lee WY, Lee KH. Song H, et al. Stem Cells Int. 2022 May 31;2022:4755514. doi: 10.1155/2022/4755514. eCollection 2022. Stem Cells Int. 2022. PMID: 35685306 Free PMC article. Review.
Isolation and Characterization of Highly Pure Type A Spermatogonia From Sterlet (Acipenser ruthenus) Using Flow-Cytometric Cell Sorting.
Xie X, Tichopád T, Kislik G, Langerová L, Abaffy P, Šindelka R, Franěk R, Fučíková M, Steinbach C, Shah MA, Šauman I, Chen F, Pšenička M. Xie X, et al. Front Cell Dev Biol. 2021 Dec 10;9:772625. doi: 10.3389/fcell.2021.772625. eCollection 2021. Front Cell Dev Biol. 2021. PMID: 34957105 Free PMC article.
Spermatogonial Stem Cells in Fish: Characterization, Isolation, Enrichment, and Recent Advances of In Vitro Culture Systems.
Xie X, Nóbrega R, Pšenička M. Xie X, et al. Biomolecules. 2020 Apr 22;10(4):644. doi: 10.3390/biom10040644. Biomolecules. 2020. PMID: 32331205 Free PMC article. Review.
Advancing aquaculture: Production of xenogenic catfish by transplanting blue catfish (Ictalurus furcatus) and channel catfish (I. punctatus) stem cells into white catfish (Ameiurus catus) triploid fry.
Hettiarachchi DU, Alston VN, Bern L, Al-Armanazi J, Su B, Shang M, Wang J, Xing D, Li S, Litvak MK, Dunham RA, Butts IAE. Hettiarachchi DU, et al. PLoS One. 2024 Jun 7;19(6):e0302687. doi: 10.1371/journal.pone.0302687. eCollection 2024. PLoS One. 2024. PMID: 38848398 Free PMC article.
Testicular germ line cell identification, isolation, and transplantation in two North American catfish species.
Shang M, Su B, Perera DA, Alsaqufi A, Lipke EA, Cek S, Dunn DA, Qin Z, Peatman E, Dunham RA. Shang M, et al. Fish Physiol Biochem. 2018 Apr;44(2):717-733. doi: 10.1007/s10695-018-0467-3. Epub 2018 Jan 22. Fish Physiol Biochem. 2018. PMID: 29357082

See all "Cited by" articles

References

1. Proc Natl Acad Sci U S A. 2006 Feb 21;103(8):2725-9 - PubMed
1. Biol Reprod. 2007 Jan;76(1):130-41 - PubMed
1. PLoS One. 2013 Jul 12;8(7):e68452 - PubMed
1. Arch Histol Cytol. 2004 Nov;67(4):285-96 - PubMed
1. J Stem Cell Res Ther. 2011 Nov 11;2(2):null - PubMed

Publication types

Actions
Actions

MeSH terms

Substances

Actions

LinkOut - more resources

Full Text Sources
- Springer
Miscellaneous
- NCI CPTAC Assay Portal

[1] Proc Natl Acad Sci U S A. 2006 Feb 21;103(8):2725-9 - PubMed

[2] Proc Natl Acad Sci U S A. 2006 Feb 21;103(8):2725-9 - PubMed

[3] Biol Reprod. 2007 Jan;76(1):130-41 - PubMed

[4] Biol Reprod. 2007 Jan;76(1):130-41 - PubMed

[5] PLoS One. 2013 Jul 12;8(7):e68452 - PubMed

[6] PLoS One. 2013 Jul 12;8(7):e68452 - PubMed

[7] Arch Histol Cytol. 2004 Nov;67(4):285-96 - PubMed

[8] Arch Histol Cytol. 2004 Nov;67(4):285-96 - PubMed

[9] J Stem Cell Res Ther. 2011 Nov 11;2(2):null - PubMed

[10] J Stem Cell Res Ther. 2011 Nov 11;2(2):null - PubMed

Save citation to file

Email citation

Add to Collections

Add to My Bibliography

Your saved search

Create a file for external citation management software

Your RSS Feed

Spermatogonial stem cells specific marker identification in channel catfish, Ictalurus punctatus and blue catfish, I. furcatus

Affiliations

Spermatogonial stem cells specific marker identification in channel catfish, Ictalurus punctatus and blue catfish, I. furcatus

Authors

Affiliations

Abstract

Similar articles

Cited by

References

Publication types

MeSH terms

Substances

LinkOut - more resources

Full Text Sources

Miscellaneous

Abstract

Similar articles

Cited by

References

Publication types

MeSH terms

Substances

Related information

LinkOut - more resources

Full Text Sources

Miscellaneous