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. 2015:2015:746856.
doi: 10.1155/2015/746856. Epub 2015 May 31.

PTEN sequence analysis in endometrial hyperplasia and endometrial carcinoma in Slovak women

H Gbelcová  1 , P Bakeš  2 , P Priščáková  2 , V Šišovský  3 , I Hojsíková  4 , Ľ Straka  5 , M Konečný  6 , J Markus  6 , C W D'Acunto  7 , T Ruml  7 , D Böhmer  2 , Ľ Danihel  3 , V Repiská  2
Affiliations

PTEN sequence analysis in endometrial hyperplasia and endometrial carcinoma in Slovak women

H Gbelcová et al. Anal Cell Pathol (Amst). 2015.

Abstract

Phosphatase and tensin homolog (PTEN) is a protein that acts as a tumor suppressor by dephosphorylating the lipid second messenger phosphatidylinositol 3,4,5-trisphosphate. Loss of PTEN function has been implicated in the pathogenesis of a number of different tumors, particularly endometrial carcinoma (ECa). ECa is the most common neoplasia of the female genital tract. Our study evaluates an association between the morphological appearance of endometrial hyperplasia and endometrial carcinoma and the degree of PTEN alterations. A total of 45 endometrial biopsies from Slovak women were included in present study. Formalin-fixed and paraffin-embedded tissue samples with simple hyperplasia (3), complex hyperplasia (5), atypical complex hyperplasia (7), endometrioid carcinomas G1 (20) and G3 (5), and serous carcinoma (5) were evaluated for the presence of mutations in coding regions of PTEN gene, the most frequently mutated tumor suppressor gene in endometrial carcinoma. 75% of the detected mutations were clustered in exons 5 and 8. Out of the 39 mutations detected in 24 cases, 20 were frameshifts and 19 were nonsense, missense, or silent mutations. Some specimens harboured more than one mutation. The results of current study on Slovak women were compared to a previous study performed on Polish population. The two sets of results were similar.

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Figures

Figure 1
Figure 1
Spectrum of PTEN mutations in Slovak women.
Figure 2
Figure 2
DNA sequencing histograms from three patients with the most frequent mutations of the PTEN gene. (a) Substitution in codon 130, G > T, forward primer used; (b) Substitution in codon 130, G > C, forward primer used; (c) A insertion in codon 117, forward primer used; (d) A deletion in codon 323, reverse primer used.
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References

    1. Jemal A., Siegel R., Xu J., Ward E. Cancer statistics, 2010. CA Cancer Journal for Clinicians. 2010;60(5):277–300. doi: 10.3322/caac.20073. - DOI - PubMed
    1. Diba C. S., Pleško I., Hlava P., editors. Incidence of Malignant Cancer in Slovak Republic 2007. Bratislava, Slovakia: National Cancer Registry of Slovak Republic, National Health Information Center; 2012.
    1. Bokhman J. V. Two pathogenetic types of endometrial carcinoma. Gynecologic Oncology. 1983;15(1):10–17. doi: 10.1016/0090-8258(83)90111-7. - DOI - PubMed
    1. Mutter G. L., Lin M.-C., Fitzgerald J. T., et al. Altered PTEN expression as a diagnostic marker for the earliest endometrial precancers. Journal of the National Cancer Institute. 2000;92(11):924–931. doi: 10.1093/jnci/92.11.924. - DOI - PubMed
    1. Li J., Yen C., Liaw D., et al. PTEN, a putative protein tyrosine phosphatase gene mutated in human brain, breast, and prostate cancer. Science. 1997;275(5308):1943–1947. doi: 10.1126/science.275.5308.1943. - DOI - PubMed

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