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Review
. 2015 Feb;3(1):AID-0027-2014.
doi: 10.1128/microbiolspec.AID-0027-2014.

Use of Human Hybridoma Technology To Isolate Human Monoclonal Antibodies

Scott A Smith  1 , James E Crowe Jr  2
Affiliations
Review

Use of Human Hybridoma Technology To Isolate Human Monoclonal Antibodies

Scott A Smith et al. Microbiol Spectr. 2015 Feb.

Abstract

The human hybridoma technique offers an important approach for isolation of human monoclonal antibodies. A diversity of approaches can be used with varying success. Recent technical advances in expanding the starting number of human antigen-specific B cells, improving fusion efficiency, and isolating new myeloma partners and new cell cloning methods have enabled the development of protocols that make the isolation of human monoclonal antibodies from blood samples feasible. Undoubtedly, additional innovations that could improve efficiency are possible.

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Conflict of interest statement

Conflicts of interest: We disclose no conflicts.

Figures

FIGURE 1
FIGURE 1
Lymphoblastoid cell formation from PBMCs. doi:10.1128/microbiolspec.AID-0027-2014.f1
FIGURE 2
FIGURE 2
(A) Pre- and (B) post-pearl chain formation. doi:10.1128/microbiolspec.AID-0027-2014.f2
FIGURE 3
FIGURE 3
Cloning in (A) semi-solid medium and (B) final human hybridoma. doi:10.1128/microbiolspec.AID-0027-2014.f3
See this image and copyright information in PMC

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