Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2015 Mar 15;8(3):4204-11.
eCollection 2015.

A proteomic study of the differential protein expression in MDBK cells after bovine herpesvirus type 1 infection (BHV-1) strain treatment

Li Guo  1 , Yanling Yang  1 , Linna Liu  2 , Peng Liao  3 , Yongjun Wen  1 , Hua Wu  1 , Shipeng Cheng  1
Affiliations

A proteomic study of the differential protein expression in MDBK cells after bovine herpesvirus type 1 infection (BHV-1) strain treatment

Li Guo et al. Int J Clin Exp Med. .

Abstract

Different BHV-1 strains, such as the virulent IBRV LN01/08 strains and the attenuated vaccine strain IBRV LNM, produces different clinical immune responses; however, the study of the differential protein expression in Madin-Darby bovine kidney (MDBK) cells after BHV-1-infection still remains unclear. Here, we applied a comparative proteomic strategy, based on 2D and MALDI-TOF/MS platforms, to examine the differential expression of proteins in MDBK cells that were treated and not treated with virulent IBRV LN01/08 and attenuated IBRV LNM strains. A total of eight differential proteins, including pyruvate kinase, heat shock protein (HSP) 90 (HSP90AA1 and HSP90AB1), annexin A, albumin (ALB), scinderin (SCIN), tubulin (alpha 1a) and vimentin (VIM), were identified. Among these proteins, pyruvate kinase, and HSP90 (HSP90AB1), tubulin and vimentin were identified in the virulent IBRV LN01/08 strain group, but were not identified in the attenuated IBRV LNM group. These results play an important role in tumor formation and development, cell migration, tumor cell line apoptosis, cell invasion and viral infection. The HSP90 (HSP90AA1) protein was identified in the control group and the attenuated IBRV LNM-infected group. Most studies have shown that HSP90 proteins were more of a cancer gene target, and inhibiting its function would result to oncogene degradation during cancer treatment. On the other hand, ALB is associated to cell differentiation, apoptosis, necrosis, cell death, viral infection, autophagy, interstitial tissue inflammation, and cell survival. These results provide a theoretical basis for the systematic understanding of BHV-1-infection mechanisms and BHV-1-induced immune responses.

Keywords: BHV-1; MDBK; comparative proteome.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Proteomic analysis of MDBK cells from the control and experimental groups (36 hours of post-exposure) using 2-DE gels. Proteins on the 2-DE gels of the experimental group (A, B) and control group (C) were visualized using Coomassie® Blue Staining.
Figure 2
Figure 2
The differential expression of proteins, which were analyzed using PDQuest. PDQuest software (BIO-RAD) was used to analyze the scanned 2-DE gel image. Spots representing different significant protein expressions were selected (Table 1) for further mass spectrometric analysis.
See this image and copyright information in PMC

Similar articles

See all similar articles

References

    1. Muylkens B, Thiry J, Kirten P, Schynts F, Thiry E. Bovine herpesvirus infection and infectious bovine rhinotracheitis. Vet Res. 2007;38:181–209. - PubMed
    1. Nandi S, Kumar M, Manohar M, Chauhan RS. Bovine herpes virus infections in cattle. Anim Health Res Rev. 2009;10:85–98. - PubMed
    1. Harrison SC. Principles of virus structure. Chapter 3. In: Knipe DM, Howley PM, editors. Fields’ virology. 4th edition. Volume 1. Philadelphia: Lippincott Williams & Wilkins; 2011. pp. 53–85.
    1. Mayfield JE, Good PJ, Vanoort HJ, Campbell AR, Reed DE. Cloning and cleavage site mapping of DNA from bovine herpesvirus 1 (cooper strain) J Virol. 1983;47:259–264. - PMC - PubMed
    1. Wyler R, Engles M, Schwyzer M. Infectious bovine rhinotracheitis/vulvovaginitis (BHV-1) In: Wittmann G, editor. Herpesvirus diseases of cattle, horses and pigs. Developments In Veterinary Virology. Boston: Kluwer Academic Publishers; 1989. pp. 1–172.

LinkOut - more resources