Analysis of caffeine and paraxanthine in human saliva with ultra-high-performance liquid chromatography for CYP1A2 phenotyping
- PMID: 26038236
- DOI: 10.1016/j.jchromb.2015.05.020
Analysis of caffeine and paraxanthine in human saliva with ultra-high-performance liquid chromatography for CYP1A2 phenotyping
Abstract
Cytochrome P450 1A2 (CYP1A2) plays an important role in drug metabolism. Caffeine (CAF) is converted into paraxanthine (PX) by this enzyme and is used as a xenobiotic substrate to determine the CYP1A2 phenotype in humans. A method for the quantification of CAF and PX in saliva was developed using liquid-liquid extraction with ethyl acetate and analysis with ultra-high-performance liquid chromatography. Peaks from CAF, PX and internal standard were resolved within 6min. The method was validated from 0.05 to 5μgmL(-1) CAF and 0.025-2.5μgmL(-1) PX. Inter- and intra-day accuracies ranged from 91.2 to 107.2% with precisions <13.5%. The limits of detection were 0.16 and 0.63 ngmL(-1) for PX and CAF, respectively. PX/CAF concentration ratios from volunteers were 0.26-1.09 with mean ratios of 0.78±0.26 and 0.38±0.10 for regular and light/non-coffee drinkers, respectively.
Keywords: CYP1A2; Caffeine; Paraxanthine; Phenotyping; Saliva; UHPLC.
Copyright © 2015 Elsevier B.V. All rights reserved.
Similar articles
-
Caffeine and paraxanthine HPLC assay for CYP1A2 phenotype assessment using saliva and plasma.Biomed Chromatogr. 2010 Oct;24(10):1136-44. doi: 10.1002/bmc.1419. Biomed Chromatogr. 2010. PMID: 20853468 Clinical Trial.
-
Validation of a high-performance liquid chromatography assay for quantification of caffeine and paraxanthine in human serum in the context of CYP1A2 phenotyping.Biomed Chromatogr. 1999 Jun;13(4):309-14. doi: 10.1002/(SICI)1099-0801(199906)13:4<309::AID-BMC881>3.0.CO;2-J. Biomed Chromatogr. 1999. PMID: 10416066
-
Development and validation of a reversed-phase HPLC method for CYP1A2 phenotyping by use of a caffeine metabolite ratio in saliva.Biomed Chromatogr. 2015 Nov;29(11):1657-63. doi: 10.1002/bmc.3475. Epub 2015 Apr 19. Biomed Chromatogr. 2015. PMID: 25891161
-
Assessment of CYP1A2 activity in clinical practice: why, how, and when?Basic Clin Pharmacol Toxicol. 2005 Sep;97(3):125-34. doi: 10.1111/j.1742-7843.2005.pto_973160.x. Basic Clin Pharmacol Toxicol. 2005. PMID: 16128905 Review.
-
[On the possibility of patient phenotyping on the basis of cytochrome p-450 1A2 isoenzyme activity using caffeine as the test substrate].Eksp Klin Farmakol. 2009 Sep-Oct;72(5):61-5. Eksp Klin Farmakol. 2009. PMID: 19928580 Review. Russian.
Cited by
-
Simultaneous Quantification of Antioxidants Paraxanthine and Caffeine in Human Saliva by Electrochemical Sensing for CYP1A2 Phenotyping.Antioxidants (Basel). 2020 Dec 24;10(1):10. doi: 10.3390/antiox10010010. Antioxidants (Basel). 2020. PMID: 33374269 Free PMC article.
-
HPLC Method for Quantification of Caffeine and Its Three Major Metabolites in Human Plasma Using Fetal Bovine Serum Matrix to Evaluate Prenatal Drug Exposure.J Anal Methods Chem. 2018 Aug 12;2018:2085059. doi: 10.1155/2018/2085059. eCollection 2018. J Anal Methods Chem. 2018. PMID: 30186659 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical
