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. 2015 Jul;160(7):1831-6.
doi: 10.1007/s00705-015-2447-6. Epub 2015 May 12.

Genomic characterization of two novel polyomaviruses in Brazilian insectivorous bats

Francisco Esmaile de Sales Lima  1 , Samuel Paulo Cibulski, André Alberto Witt, Ana Cláudia Franco, Paulo Michel Roehe
Affiliations

Genomic characterization of two novel polyomaviruses in Brazilian insectivorous bats

Francisco Esmaile de Sales Lima et al. Arch Virol. 2015 Jul.

Abstract

Two novel genomes comprising ≈4.9 kb were identified by next-generation sequencing from pooled organs of Tadarida brasiliensis bats. The overall nucleotide sequence identities between the viral genomes characterized here were less than 80% in comparison to other polyomaviruses (PyVs), members of the family Polyomaviridae. The new genomes display the archetypal organization of PyVs, which includes open reading frames for the regulatory proteins small T antigen (STAg) and large T antigen (LTAg), as well as capsid proteins VP1, VP2 and VP3. In addition, an alternate ORF was identified in the early genome region that is conserved in a large monophyletic group of polyomaviruses. Phylogenetic analysis showed similar clustering with group of PyVs detected in Otomops and Chaerephon bats and some species of monkeys. In this study, the genomes of two novel PyVs were detected in bats of a single species, demonstrating that these mammals can harbor genetically diverse polyomaviruses.

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Figures

Fig. 1
Fig. 1
Schematic diagram showing the genome organization of Tadarida brasiliensis polyomaviruses 1 and 2 (TbPyV1 and TvPyV2). Putative coding regions for VP1 to VP3, small T antigen (STAg), large T antigen (LTAg), and the alternate large T open reading frame (ALTO) are marked by arrows
Fig. 2
Fig. 2
Bayesian phylogenetic trees of the large and small T antigens, the major capsid protein VP1, and the minor capsid proteins VP2/VP3 of the novel bat PyV genomes. Amino acid sequences were compared to those of 28 polyomaviruses from mammals and birds, retrieved from GenBank. Posterior probability values are indicated above the branches. In these phylogenies, well-supported clades that contained the bat PyVs are shaded in gray
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