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. 2015 Jul 17;10(7):1648-53.
doi: 10.1021/acschembio.5b00230. Epub 2015 May 12.

Incorporation of Unnatural Amino Acids in Response to the AGG Codon

Byeong Sung Lee, Seunggun Shin, Jong Yeob Jeon, Kyoung-Soon Jang  1 , Bun Yeol Lee, Sangdun Choi, Tae Hyeon Yoo
Affiliations

Incorporation of Unnatural Amino Acids in Response to the AGG Codon

Byeong Sung Lee et al. ACS Chem Biol. .

Abstract

The biological protein synthesis system has been engineered to incorporate unnatural amino acid into proteins, and this has opened up new routes for engineering proteins with novel compositions. While such systems have been successfully applied in research, there remains a need to develop new approaches with respect to the wider application of unnatural amino acids. In this study, we reported a strategy for incorporating unnatural amino acids into proteins by reassigning one of the Arg sense codons, the AGG codon. Using this method, several unnatural amino acids were quantitatively incorporated into the AGG site. Furthermore, we applied the method to multiple AGG sites, and even to tandem AGG sequences. The method developed and described here could be used for engineering proteins with diverse unnatural amino acids, particularly when employed in combination with other methods.

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