Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2015 Mar 24:6:128.
doi: 10.3389/fpls.2015.00128. eCollection 2015.

Proteome readjustments in the apoplastic space of Arabidopsis thaliana ggt1 mutant leaves exposed to UV-B radiation

Anna Rita Trentin  1 , Micaela Pivato  2 , Syed M M Mehdi  1 , Leonard Ebinezer Barnabas  3 , Sabrina Giaretta  1 , Marta Fabrega-Prats  1 , Dinesh Prasad  4 , Giorgio Arrigoni  5 , Antonio Masi  1
Affiliations

Proteome readjustments in the apoplastic space of Arabidopsis thaliana ggt1 mutant leaves exposed to UV-B radiation

Anna Rita Trentin et al. Front Plant Sci. .

Abstract

Ultraviolet-B radiation acts as an environmental stimulus, but in high doses it has detrimental effects on plant metabolism. Plasma membranes represent a major target for Reactive Oxygen Species (ROS) generated by this harmful radiation. Oxidative reactions occurring in the apoplastic space are counteracted by antioxidative systems mainly involving ascorbate and, to some extent, glutathione. The occurrence of the latter and its exact role in the extracellular space are not well documented, however. In Arabidopsis thaliana, the gamma-glutamyl transferase isoform (GGT1) bound to the cell wall takes part in the so-called gamma-glutamyl cycle for extracellular glutathione degradation and recovery, and may be implicated in redox sensing and balance. In this work, oxidative conditions were imposed with Ultraviolet-B radiation (UV-B) and studied in redox altered ggt1 mutants. The response of ggt1 knockout Arabidopsis leaves to UV-B radiation was assessed by investigating changes in extracellular glutathione and ascorbate content and their redox state, and in apoplastic protein composition. Our results show that, on UV-B exposure, soluble antioxidants respond to the oxidative conditions in both genotypes. Rearrangements occur in their apoplastic protein composition, suggesting an involvement of Hydrogen Peroxide (H2O2), which may ultimately act as a signal. Other important changes relating to hormonal effects, cell wall remodeling, and redox activities are discussed. We argue that oxidative stress conditions imposed by UV-B and disruption of the gamma-glutamyl cycle result in similar stress-induced responses, to some degree at least. Data are available via ProteomeXchange with identifier PXD001807.

Keywords: apoplast; gamma-glutamyl-transferase; glutathione; iTRAQ labeling; oxidative stress; ultraviolet-B radiation.

PubMed Disclaimer

Figures

Figure 1
Figure 1
GGT activity (A), glutathione (B), ascorbate (C) and cys-gly (D) in ECWF. Gray bars show total content, white bars oxidized forms. Reported values are the mean ± S.E. of 3 technical replicates, each conducted with at least 4 biological replicates. Different letters indicate significant differences between conditions (P = 0.05*; P = 0.01**; P = 0.001***). For GGT activity, the reference value of the wild type control was 43.05 mU/mL ECWF.
Figure 2
Figure 2
GO terms distribution in the biological process of downregulated proteins. Black bars shows wt (UV-B/ctrl), dark gray bars ggt1 (UV-B/ ctrl), light gray is ctrl (ggt1/wt), and white bars is UV-B (ggt1/wt).
Figure 3
Figure 3
GO terms distribution in the biological process of upregulated proteins. Black bars shows wt (UV-B/ctrl), dark gray bars ggt1 (UV-B/ ctrl), light gray is ctrl (ggt1/wt), and white bars is UV-B (ggt1/wt).
Figure 4
Figure 4
Schematic overview of apoplastic proteome variations in: (A) wild type, induced by UV-B; (B) ggt1 genotype due to the mutation and/or to UV-B treatment. Vertical arrows refer to stimulation (↑) or repression (↓) caused by the mutation; horizontal arrows indicate repression (⊢) or stimulation (←) caused by UV-B treatment.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Agrawal G. K., Jwa N. S., Lebrun M. H., Job D., Rakwal R. (2010). Plant secretome: unlocking secrets of the secreted proteins. Proteomics 10, 799–827. 10.1002/pmic.200900514 - DOI - PubMed
    1. Alonso J. M., Stepanova A. N., Leisse T. J., Kim C. J., Chen H., Shinn P., et al. . (2003). Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301, 653–657. 10.1126/science.1086391 - DOI - PubMed
    1. Alscher R. G., Erturk N., Health L. S. (2002). Role of superoxide dismutases (SODs) in controlling oxidative stress in plants. J. Exp. Bot. 53, 1331–1341. 10.1093/jexbot/53.372.1331 - DOI - PubMed
    1. Bienert G. P., Schjoerring J. K., Jahn T. P. (2006). Membrane transport of hydrogen peroxide. Biochim. Biophys. Acta 1758, 994–1004. 10.1016/j.bbamem.2006.02.015 - DOI - PubMed
    1. Brandes N., Schmitt S., Jakob U. (2009). Thiol-based redox switches in eukaryotic proteins. Antiox. Redox Signal. 11, 997–1014 10.1089/ars.2008.2285 - DOI - PMC - PubMed

LinkOut - more resources