Effects of pH and Temperature on Antibacterial Activity of Zinc Oxide Nanofluid Against Escherichia coli O157: H7 and Staphylococcus aureus
- PMID: 25825643
- PMCID: PMC4376975
- DOI: 10.5812/jjm.17115
Effects of pH and Temperature on Antibacterial Activity of Zinc Oxide Nanofluid Against Escherichia coli O157: H7 and Staphylococcus aureus
Abstract
Background: Zinc oxide nanoparticles (ZnO NPs) are known as one of the important inorganic materials used in research and health-related applications with effective antibacterial activities. Although the toxic effects of ZnO NPs have already been evaluated, more information is required to understand the possible mechanisms.
Objectives: The aim of the present study was to determine the influences of pH and temperature on antibacterial activity of ZnO NPs against some strains of pathogenic bacteria. Identifying the interrelationship between toxicity and cultural conditions helps us to have a better understanding of the optimum reaction conditions for maximum antimicrobial activity.
Materials and methods: ZnO NPs were prepared and characterized and then dispersed in glycerol with the help of ammonium citrate as the dispersant. The antibacterial tests were performed by measuring the growth of Escherichia coli O157:H7 and Staphylococcus aureus with different concentrations of ZnO NPs in glycerol. All the experiments were conducted at different incubation temperatures (25-42(°)C) and pH levels (4-10 for E. coli O157:H7 and 5-10 for S. aureus).
Results: The results showed that ZnO nanofluid have antibacterial activity against E. coli O157:H7 and S. aureus and the inhibitory effect increases with increasing the nanofluid concentration. The experiments showed that the antibacterial activity of ZnO NPs was influenced by temperature and pH. Higher antibacterial activity was observed at acidic pH levels with the maximum toxicity at pH = 4 and pH = 5 for E. coli O157: H7 and S. aureus, respectively. By raising the temperature, the toxicity of ZnO nanofluid increased, with the highest antibacterial activity at 42°C for both bacterial types in comparison with positive controls under the same conditions.
Conclusions: Analysis of the results demonstrated that exposure media of ZnO NPs and cultural factors play a role in their cytotoxic effects. It could be attributed to the principal mechanism at different reaction conditions.
Keywords: Escherichia coli O157: H7; Nanoparticles; Staphylococcus aureus; Treatment.
Figures
Similar articles
-
Antibacterial activities of zinc oxide nanoparticles against Escherichia coli O157:H7.J Appl Microbiol. 2009 Oct;107(4):1193-201. doi: 10.1111/j.1365-2672.2009.04303.x. Epub 2009 Apr 17. J Appl Microbiol. 2009. PMID: 19486396
-
Antibacterial activity of poly-l-arginine under different conditions.Iran J Microbiol. 2017 Apr;9(2):103-111. Iran J Microbiol. 2017. PMID: 29214002 Free PMC article.
-
Antibacterial Activities of Wasabi against Escherichia coli O157:H7 and Staphylococcus aureus.Front Microbiol. 2016 Sep 21;7:1403. doi: 10.3389/fmicb.2016.01403. eCollection 2016. Front Microbiol. 2016. PMID: 27708622 Free PMC article.
-
ZnO Nanomaterials: Current Advancements in Antibacterial Mechanisms and Applications.Front Chem. 2020 Jul 21;8:580. doi: 10.3389/fchem.2020.00580. eCollection 2020. Front Chem. 2020. PMID: 32793554 Free PMC article. Review.
-
Nanomaterials in the Management of Gram-Negative Bacterial Infections.Nanomaterials (Basel). 2021 Sep 28;11(10):2535. doi: 10.3390/nano11102535. Nanomaterials (Basel). 2021. PMID: 34684977 Free PMC article. Review.
Cited by
-
A narrative review on application of metal and metal oxide nanoparticles in endodontics.Heliyon. 2024 Jul 16;10(15):e34673. doi: 10.1016/j.heliyon.2024.e34673. eCollection 2024 Aug 15. Heliyon. 2024. PMID: 39145007 Free PMC article. Review.
-
Alginate and Chitosan-Based Hydrogel Enhance Antibacterial Agent Activity on Topical Application.Infect Drug Resist. 2024 Mar 1;17:791-805. doi: 10.2147/IDR.S456403. eCollection 2024. Infect Drug Resist. 2024. PMID: 38444772 Free PMC article. Review.
-
Synthesis, Characterization, and Ecotoxicology Assessment of Zinc Oxide Nanoparticles by In Vivo Models.Nanomaterials (Basel). 2024 Jan 24;14(3):255. doi: 10.3390/nano14030255. Nanomaterials (Basel). 2024. PMID: 38334526 Free PMC article.
-
Alternative mechanisms of action of metallic nanoparticles to mitigate the global spread of antibiotic-resistant bacteria.Cell Surf. 2023 Oct 20;10:100112. doi: 10.1016/j.tcsw.2023.100112. eCollection 2023 Dec 15. Cell Surf. 2023. PMID: 37920217 Free PMC article. Review.
-
The Development of New Nanocomposite Polytetrafluoroethylene/Fe2O3 NPs to Prevent Bacterial Contamination in Meat Industry.Polymers (Basel). 2022 Nov 12;14(22):4880. doi: 10.3390/polym14224880. Polymers (Basel). 2022. PMID: 36433009 Free PMC article.
References
-
- Tayel AA, El-Tras WF, Moussa S, El-Baz AF, Mahrous H, Salem MF, et al. Antibacterial Action of Zinc Oxide Nanoparticles against Foodborne Pathogens. J Food Safety. 2011;31(2):211–8. doi: 10.1111/j.1745-4565.2010.00287.x. - DOI
LinkOut - more resources
Full Text Sources
Other Literature Sources