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. 2012 Sep;15(3):19-30.
doi: 10.3831/KPI.2012.15.002.

Galectin-3-independent Down-regulation of GABABR1 due to Treatment with Korean Herbal Extract HAD-B Reduces Proliferation of Human Colon Cancer Cells

Kyung-Hee Kim  1 , Yong-Kyun Kwon  2 , Chong-Kwan Cho  2 , Yeon-Weol Lee  2 , So-Hyun Lee  3 , Sang-Geun Jang  1 , Byong-Chul Yoo  3 , Hwa-Seong Yoo  2
Affiliations

Galectin-3-independent Down-regulation of GABABR1 due to Treatment with Korean Herbal Extract HAD-B Reduces Proliferation of Human Colon Cancer Cells

Kyung-Hee Kim et al. J Pharmacopuncture. 2012 Sep.

Abstract

Objectives: Many efforts have shown multi-oncologic roles of galectin-3 for cell proliferation, angiogenesis, and apoptosis. However, the mechanisms by which galectin-3 is involved in cell proliferation are not yet fully understood, especially in human colon cancer cells.

Methods: To cluster genes showing positively or negatively correlated expression with galectin-3, we employed human colon cancer cell lines, SNU-61, SNU-81, SNU-769B, SNU-C4 and SNU-C5 in high-throughput gene expression profiling. Gene and protein expression levels were determined by using real-time quantitative polymerase chain reaction (PCR) and western blot analysis, respectively. The proliferation rate of human colon cancer cells was measured by using a 3-(4, 5- dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay.

Results: Expression of γ-aminobutyric acid B receptor 1 (GABABR1) showed a positive correlation with galectin-3 at both the transcriptional and the translational levels. Downregulation of galectin-3 decreased not only GABABR1 expression but also the proliferation rate of human colon cancer cells. However, Korean herbal extract, HangAmDan-B (HAD-B), decreased expression of GABABR1 without any expressional change of galectin-3, and offset γ-aminobutyric acid (GABA)-enhanced human colon cancer cell proliferation.

Conclusions: Our present study confirmed that GABABR1 expression was regulated by galectin-3. HAD-B induced galectin-3-independent down-regulation of GABABR1, which resulted in a decreased proliferation of human colon cancer cells. The therapeutic effect of HAD-B for the treatment of human colon cancer needs to be further validated.

Keywords: 5-fluorouracil; GABABR1; HAD-B; galectin-3; human colon cancer; proliferation.

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Figures

Fig. 1
Fig. 1. Galectin-3 expression correlated with 5-FU susceptibility in human colon cancer cell lines and gene expression profiling linked to galectin-3.
Fig. 2
Fig. 2. Gene and protein expressions of GABABR1 positively linked to galectin-3 expression.
Fig. 3
Fig. 3. Reduced GABABR1 expression and suppressed cell proliferation of SNU-C4 by treatment with HAD-B.
See this image and copyright information in PMC

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