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. 2015:1271:97-111.
doi: 10.1007/978-1-4939-2330-4_7.

Detection of structural waters and their role in structural dynamics of rhodopsin activation

Liwen Wang  1 , Mark R Chance
Affiliations

Detection of structural waters and their role in structural dynamics of rhodopsin activation

Liwen Wang et al. Methods Mol Biol. 2015.

Abstract

Conserved structural waters trapped within GPCRs may form water networks indispensable for GPCR's signaling functions. Radiolysis-based hydroxyl radical footprinting (HRF) strategies coupled to mass spectrometry have been used to explore the structural waters within rhodopsin in multiple signaling states. These approaches, combined with (18)O labeling, can be used to identify the locations of structural waters in the transmembrane region and measure rates of water exchange with bulk solvent. Reorganizations of structural waters upon activation of signaling can be explicitly observed with this approach, and this provides a unique look at the structural modules driving the signaling process.

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