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. 2015:2015:208514.
doi: 10.1155/2015/208514. Epub 2015 Jan 27.

Effect of GuiXiong Xiaoyi Wan in Treatment of Endometriosis on Rats

Zhixing Jin  1 , Li Wang  2 , Zhiling Zhu  2
Affiliations

Effect of GuiXiong Xiaoyi Wan in Treatment of Endometriosis on Rats

Zhixing Jin et al. Evid Based Complement Alternat Med. 2015.

Abstract

Objective. To evaluate the effect of GuiXiong Xiaoyi Wan (GXXYW) on the development of endometriosis in a rat model. Methods. Sprague-Dawley rats with surgically induced endometriosis were randomly treated with low-dose GXXYW, high-dose GXXYW, or vehicle (negative control) for 28 days. Immunohistochemistry was used to assess cell proliferation in the lesions. The terminal deoxynucleotidyl transferase- (TdT-) mediated dUTP biotin nick end labelling (TUNEL) method was performed to analyse the apoptosis induced by GuiXiong Xiaoyi Wan. The percentages of CD3+ lymphocytes, CD4+ lymphocytes, and CD8+ lymphocytes in the spleens of the rats were evaluated using flow cytometric analysis. Results. Treatment with GXXYW significantly decreased the lesion size, inhibited cell proliferation, and induced apoptosis in endometriotic tissue. The spleens of GXXYW-treated rats also demonstrated a significant increase in the percentage of CD4+ lymphocytes and a significant decrease in the percentage of CD8+ lymphocytes. Conclusions. These results suggest that, in a rat model, GXXYW may be effective in the suppression of the growth of endometriosis, possibly through the inhibition of cell proliferation, the induction of apoptosis of endometriotic cells, and the regulation of the immune system.

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Figures

Figure 1
Figure 1
The volume of ectopic endometrial tissue after the treatment with different doses of the GXXYW via oral gavage. (a) Comparison of the volume of the lesion size in each group after 28 days of treatment; (b) Box plot showing endometrial volumes of different groups. Rats in the control group received distilled water only; rats in the low-dose group were administered GXXYW 13 g/kg/day; rats in the high-dose group were administered GXXYW 26 g/kg/day.
Figure 2
Figure 2
Effect of GXXYW on cell proliferation in endometriotic lesions. Cell proliferation was evaluated by immunohistochemistry for the expression of proliferating cell nuclear antigen (PCNA). (a) Comparison of the percentage of PCNA+ cells in the different dose groups after 28 days of treatment; 400x Magnification. (b) Box plot showing the percentage of PCNA+ cells. Control group received distilled water only; low dose, GXXYW low-dose group; high dose, GXXYW high-dose group.
Figure 3
Figure 3
TUNEL assay of ectopic endometrial tissue in the different groups (200x magnification).
Figure 4
Figure 4
Box plots showing treatment-related enhancement of a cell-mediated immune response in the spleens of experimental rats. Splenocytes were isolated and analysed by flow cytometry for the expression of CD3+, CD4+, and CD8+ cells. (a) Box plot showing the percentage of CD3+ T cells in gate; (b) Box plot showing the percentage of CD4+ T cells in gate; (c) Box plot showing the percentage of CD8+ T cells in gate; (d) Box plot showing the ratio of CD4+ T cells/CD8+ T cells. Rats in the control group and sham group received distilled water only; rats in the low-dose group were administered GXXYW 13 g/kg/day; rats in the high-dose group were administered GXXYW 26 g/kg/day.
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