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. 2015;12(2):109-14.
doi: 10.1080/15476286.2015.1008926.

The NineTeen Complex (NTC) and NTC-associated proteins as targets for spliceosomal ATPase action during pre-mRNA splicing

Rogerio Alves de Almeida  1 , Raymond T O'Keefe
Affiliations

The NineTeen Complex (NTC) and NTC-associated proteins as targets for spliceosomal ATPase action during pre-mRNA splicing

Rogerio Alves de Almeida et al. RNA Biol. 2015.

Abstract

Pre-mRNA splicing is an essential step in gene expression that removes intron sequences efficiently and accurately to produce a mature mRNA for translation. It is the large and dynamic RNA-protein complex called the spliceosome that catalyzes intron removal. To carry out splicing the spliceosome not only needs to assemble correctly with the pre-mRNA but the spliceosome requires extensive remodelling of its RNA and protein components to execute the 2 steps of intron removal. Spliceosome remodelling is achieved through the action of ATPases that target both RNA and proteins to produce spliceosome conformations competent for each step of spliceosome activation, catalysis and disassembly. An increasing amount of research has pointed to the spliceosome associated NineTeen Complex (NTC) of proteins as targets for the action of a number of the spliceosomal ATPases during spliceosome remodelling. In this point-of-view article we present the latest findings on the changes in the NTC that occur following ATPase action that are required for spliceosome activation, catalysis and disassembly. We proposed that the NTC is one of the main targets of ATPase action during spliceosome remodelling required for pre-mRNA splicing.

Keywords: ATPase; Brr2; Cwc2; NineTeen Complex; PremRNA splicing; Prp16; Prp19; Prp2; Prp43; RNA helicase.

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Figures

Figure 1.
Figure 1.
NTC protein remodelling by ATPases during the spliceosome assembly, activation and disassembly process. The pathway of complexes formed on the pre-mRNA during spliceosome assembly, activation and disassembly are indicated with arrows and the names of each complex are given below the complex. The ATPases are shown in red under the arrow for the step that each ATPase promotes. The NTC core complex (dark orange) and NTC-associated proteins (light orange) are shown below each complex that they associate with. Arrows from the NTC complex are used to indicate the proteins that leave following ATPase action. Question marks are used to indicate that experimental evidence for Yju2 removal from the NTC and Brr2 action during spliceosome disassembly is not in agreement.
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