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. 2014;2(1):03.
doi: 10.1128/microbiolspec.MGM2-0018-2013.

Regulated Expression Systems for Mycobacteria and Their Applications

Dirk Schnappinger  1 , Sabine Ehrt  2
Affiliations

Regulated Expression Systems for Mycobacteria and Their Applications

Dirk Schnappinger et al. Microbiol Spectr. 2014.

Abstract

For bacterial model organisms like Escherichia coli and Bacillus subtilis genetic tools to experimentally manipulate the activity of individual genes existed for decades. But for genetically less tractable yet medically important bacteria such as M. tuberculosis such tools have rarely been available. More recently several groups developed genetic switches that function efficiently in M. tuberculosis and other mycobacteria. Together these systems utilize six different transcription factors, eight different regulated promoters, and three different regulatory principles. Here we describe their design features, review their main applications, and discuss advantages and disadvantages of regulating transcription, translation, or protein stability for controlling gene activities in bacteria.

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Figures

Figure 1
Figure 1. Regulatory systems for mycobacteria
The transcriptional regulatory systems are shown in (a) to (h), the two controlled proteolysis systems in (i) and (j), and the theophylline riboswitch in (k). Dotted lines ending in a perpendicular line indicate negative regulatory interactions; dotted lines ending in an arrow represent positive regulatory interactions. Ace, acetamide; tc/atc, tetracycline / anhydrotetracycline; IPTG, isopropyl β-D-1-thiogalactopyranoside; ara, arabinose; IVN, isovaleronitrile.
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