A major transcriptional regulatory protein (ICP4) of herpes simplex virus type 1 is associated with purified virions
- PMID: 2545909
- PMCID: PMC250907
- DOI: 10.1128/JVI.63.8.3338-3344.1989
A major transcriptional regulatory protein (ICP4) of herpes simplex virus type 1 is associated with purified virions
Abstract
Herpes simplex virus type 1 was purified by density gradient centrifugation, and the virion-associated proteins were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. By Western blot (immunoblot) analysis with an anti-ICP4 monospecific serum, the results indicated that ICP4, one of the five immediate-early proteins of herpes simplex virus type 1, was associated with the purified virions. To define the location of ICP4 within the virion, trypsin digestion experiments were performed. Purified virions were treated with trypsin in the presence or absence of detergent. The virus envelope appeared to protect ICP4 from the trypsin, since virus-associated ICP4 was sensitive to digestion only after detergent treatment. In addition, ICP4 remained associated with the virus particle when the virion-specific glycoproteins were removed after detergent treatment. Finally, ICP4 was not detected in purified preparations of type A and B capsids isolated from the nuclear fraction of virus-infected cells. The above-mentioned data suggest that detectable amounts of ICP4 are present within the tegument region of the virion.
Similar articles
-
Association of a major transcriptional regulatory protein, ICP4, of herpes simplex virus type 1 with the plasma membrane of virus-infected cells.J Virol. 1991 Mar;65(3):1516-24. doi: 10.1128/JVI.65.3.1516-1524.1991. J Virol. 1991. PMID: 1847468 Free PMC article.
-
Association of ICP0 but not ICP27 with purified virions of herpes simplex virus type 1.J Virol. 1992 May;66(5):2709-16. doi: 10.1128/JVI.66.5.2709-2716.1992. J Virol. 1992. PMID: 1313896 Free PMC article.
-
Influence of the host cell on the association of ICP4 and ICP0 with herpes simplex virus type 1.Virology. 1995 Aug 1;211(1):209-17. doi: 10.1006/viro.1995.1393. Virology. 1995. PMID: 7645212
-
A 165 kd protein of the herpes simplex virion shares a common epitope with the regulatory protein, ICP4.Biochem Biophys Res Commun. 1989 Aug 30;163(1):124-30. doi: 10.1016/0006-291x(89)92108-6. Biochem Biophys Res Commun. 1989. PMID: 2476124
-
Isolation of herpes simplex virus regulatory protein ICP4 as a homodimeric complex.J Virol. 1985 Aug;55(2):329-37. doi: 10.1128/JVI.55.2.329-337.1985. J Virol. 1985. PMID: 2991559 Free PMC article.
Cited by
-
Cellular Protein WDR11 Interacts with Specific Herpes Simplex Virus Proteins at the trans-Golgi Network To Promote Virus Replication.J Virol. 2015 Oct;89(19):9841-52. doi: 10.1128/JVI.01705-15. Epub 2015 Jul 15. J Virol. 2015. PMID: 26178983 Free PMC article.
-
Association of a major transcriptional regulatory protein, ICP4, of herpes simplex virus type 1 with the plasma membrane of virus-infected cells.J Virol. 1991 Mar;65(3):1516-24. doi: 10.1128/JVI.65.3.1516-1524.1991. J Virol. 1991. PMID: 1847468 Free PMC article.
-
Early Steps in Herpes Simplex Virus Infection Blocked by a Proteasome Inhibitor.mBio. 2019 May 14;10(3):e00732-19. doi: 10.1128/mBio.00732-19. mBio. 2019. PMID: 31088925 Free PMC article.
-
A specific subform of the human cytomegalovirus transactivator protein pUL69 is contained within the tegument of virus particles.J Virol. 1996 Dec;70(12):8984-7. doi: 10.1128/JVI.70.12.8984-8987.1996. J Virol. 1996. PMID: 8971028 Free PMC article.
-
Herpes simplex viral nucleoprotein creates a competitive transcriptional environment facilitating robust viral transcription and host shut off.Elife. 2019 Oct 22;8:e51109. doi: 10.7554/eLife.51109. Elife. 2019. PMID: 31638576 Free PMC article.
References
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
