Differential expression of immune-related cytokine genes in response to J group avian leukosis virus infection in vivo

doi:10.1016/j.molimm.2014.11.004

. 2015 Mar;64(1):106-11.

doi: 10.1016/j.molimm.2014.11.004. Epub 2014 Nov 29.

Differential expression of immune-related cytokine genes in response to J group avian leukosis virus infection in vivo

Yanni Gao¹, Yongzhen Liu², Xiaolu Guan³, Xiaofei Li⁴, Bingling Yun⁵, Xiaole Qi⁶, Yongqiang Wang⁷, Honglei Gao⁸, Hongyu Cui⁹, Changjun Liu¹⁰, Yanping Zhang¹¹, Xiaomei Wang¹², Yulong Gao¹³

Affiliations

¹ Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427, Maduan Street, Nan Gang District, Harbin 150001, Heilongjiang Province, PR China. Electronic address: yngao03@sina.com.
² Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427, Maduan Street, Nan Gang District, Harbin 150001, Heilongjiang Province, PR China. Electronic address: 651854815@qq.com.
³ Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427, Maduan Street, Nan Gang District, Harbin 150001, Heilongjiang Province, PR China. Electronic address: guanxiaolu07@163.com.
⁴ Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427, Maduan Street, Nan Gang District, Harbin 150001, Heilongjiang Province, PR China. Electronic address: 965232411@qq.com.
⁵ Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427, Maduan Street, Nan Gang District, Harbin 150001, Heilongjiang Province, PR China. Electronic address: yunbingling2010@126.com.
⁶ Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427, Maduan Street, Nan Gang District, Harbin 150001, Heilongjiang Province, PR China. Electronic address: qxl@hvri.ac.cn.
⁷ Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427, Maduan Street, Nan Gang District, Harbin 150001, Heilongjiang Province, PR China. Electronic address: yqw@hvri.ac.cn.
⁸ Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427, Maduan Street, Nan Gang District, Harbin 150001, Heilongjiang Province, PR China. Electronic address: ghl@hvri.ac.cn.
⁹ Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427, Maduan Street, Nan Gang District, Harbin 150001, Heilongjiang Province, PR China. Electronic address: cuihongyu@caas.cn.
¹⁰ Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427, Maduan Street, Nan Gang District, Harbin 150001, Heilongjiang Province, PR China. Electronic address: liucj93711@hvri.ac.cn.
¹¹ Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427, Maduan Street, Nan Gang District, Harbin 150001, Heilongjiang Province, PR China. Electronic address: zhangyanping03@caas.cn.
¹² Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427, Maduan Street, Nan Gang District, Harbin 150001, Heilongjiang Province, PR China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou 225009, PR China. Electronic address: xmw@hvri.ac.cn.
¹³ Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427, Maduan Street, Nan Gang District, Harbin 150001, Heilongjiang Province, PR China. Electronic address: ylg@hvri.ac.cn.

PMID: 25438822
DOI: 10.1016/j.molimm.2014.11.004

Differential expression of immune-related cytokine genes in response to J group avian leukosis virus infection in vivo

Yanni Gao et al. Mol Immunol. 2015 Mar.

. 2015 Mar;64(1):106-11.

doi: 10.1016/j.molimm.2014.11.004. Epub 2014 Nov 29.

Authors

Affiliations

¹ Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427, Maduan Street, Nan Gang District, Harbin 150001, Heilongjiang Province, PR China. Electronic address: yngao03@sina.com.
² Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427, Maduan Street, Nan Gang District, Harbin 150001, Heilongjiang Province, PR China. Electronic address: 651854815@qq.com.
³ Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427, Maduan Street, Nan Gang District, Harbin 150001, Heilongjiang Province, PR China. Electronic address: guanxiaolu07@163.com.
⁴ Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427, Maduan Street, Nan Gang District, Harbin 150001, Heilongjiang Province, PR China. Electronic address: 965232411@qq.com.
⁵ Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427, Maduan Street, Nan Gang District, Harbin 150001, Heilongjiang Province, PR China. Electronic address: yunbingling2010@126.com.
⁶ Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427, Maduan Street, Nan Gang District, Harbin 150001, Heilongjiang Province, PR China. Electronic address: qxl@hvri.ac.cn.
⁷ Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427, Maduan Street, Nan Gang District, Harbin 150001, Heilongjiang Province, PR China. Electronic address: yqw@hvri.ac.cn.
⁸ Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427, Maduan Street, Nan Gang District, Harbin 150001, Heilongjiang Province, PR China. Electronic address: ghl@hvri.ac.cn.
⁹ Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427, Maduan Street, Nan Gang District, Harbin 150001, Heilongjiang Province, PR China. Electronic address: cuihongyu@caas.cn.
¹⁰ Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427, Maduan Street, Nan Gang District, Harbin 150001, Heilongjiang Province, PR China. Electronic address: liucj93711@hvri.ac.cn.
¹¹ Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427, Maduan Street, Nan Gang District, Harbin 150001, Heilongjiang Province, PR China. Electronic address: zhangyanping03@caas.cn.
¹² Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427, Maduan Street, Nan Gang District, Harbin 150001, Heilongjiang Province, PR China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou 225009, PR China. Electronic address: xmw@hvri.ac.cn.
¹³ Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427, Maduan Street, Nan Gang District, Harbin 150001, Heilongjiang Province, PR China. Electronic address: ylg@hvri.ac.cn.

PMID: 25438822
DOI: 10.1016/j.molimm.2014.11.004

Abstract

Infection with J group avian leukosis virus (ALV-J) can result in immunosuppression and subsequently increased susceptibility to secondary infection. The innate immune system is the first line defense system in prevention of further bacterial and viral infections. Cytokines play key roles in the innate immune system. In this study, we used RT-qPCR technology to test the cytokine mRNA expression levels in various immune tissues, including the spleen, bursa of fabricius and cecal tonsil, in the days following ALV-J infection. The results indicated that in the infected group, the expression levels of interleukin-6 (IL-6), IL-18, interferon-α (IFN-α) and IFN-γ significantly increased in the spleen and reached peak levels that were thousandfolds higher than baselines at 9-12 days post-infection (d.p.i.). The levels in the bursa of fabricius slightly increased, and the levels in the cecal tonsil were not significantly altered. Moreover, the pattern of the expression of these three cytokines in the spleens of the infected group was similar to the pattern of viremia of this group. These results suggest that the spleen plays an important role in the interaction between ALV-J infection and the innate immune system. This study contributes to the understanding of innate immune responses to ALV-J infection and also elucidates the mechanisms of the pathogenicity of ALV-J in chickens.

Keywords: ALV-J; Innate immune responses; Interferon; Interleukin.

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Differential expression of immune-related cytokine genes in response to J group avian leukosis virus infection in vivo

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Differential expression of immune-related cytokine genes in response to J group avian leukosis virus infection in vivo

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