Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Observational Study
. 2014 Dec;109(8):989-98.
doi: 10.1590/0074-0276140182. Epub 2014 Nov 21.

Lymphocyte subsets in human immunodeficiency virus-unexposed Brazilian individuals from birth to adulthood

Maria Isabel de Moraes-Pinto  1 , Erika Ono  1 , Elisângela C Santos-Valente  1 , Liziane C Almeida  1 , Paula Rosemberg de Andrade  2 , Maria Isabel Saraiva Dinelli  1 , Amélia M Nunes dos Santos  3 , Reinaldo Salomão  1
Affiliations
Observational Study

Lymphocyte subsets in human immunodeficiency virus-unexposed Brazilian individuals from birth to adulthood

Maria Isabel de Moraes-Pinto et al. Mem Inst Oswaldo Cruz. 2014 Dec.

Abstract

Ethnic origin, genetics, gender and environmental factors have been shown to influence some immunologic indices, so that development of reference values for populations of different backgrounds may be necessary. We have determined the distribution of lymphocyte subsets in healthy Brazilian individuals from birth to adulthood. Lymphocyte subsets were determined using four-colour cytometry in a cross-sectional study of 463 human immunodeficiency virus-unexposed children and adults from birth through 49 years of age. Lymphocyte subsets varied according to age, as previously observed in other studies. However, total CD4+ T cell numbers were lower than what was described in the Pediatric AIDS Clinical Trials Group P1009 (PACTG P1009), which assessed an American population of predominantly African and Hispanic backgrounds until the 12-18 year age range, when values were comparable. Naïve percentages and absolute values of CD8+ T cells, as assessed by CD45RA expression, were also lower than the PACTG P1009 data for all analysed age ranges. CD38 expression on both CD4+ and CD8+ T cells was lower than the PACTG P1009 values, with a widening gap between the two studies at older age ranges. Different patterns of cell differentiation seem to occur in different settings and may have characteristic expression within each population.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1. : illustrative example of flow cytometry plots for data analysis. Lymphocytes were selected on the side scatter/forward scatter plot with a gate as shown in A. T cells were identified from the gate of lymphocytes as CD3+ (B), B cells, as CD3-CD19+ (C) and natural killer (NK) cells, as CD3-CD16+/CD56+ (D). CD4+ T cells were identified from the gate of lymphocytes as CD3+CD4+ (E) and then separated in differentiation subsets using CD45RA/CCR7 (F) or CD45RA/CD27 markers (G); CD38 and CD25 expression were evaluated on CD4+ T cells in H and I, respectively. CD8+ T cells were identified from the gate of lymphocytes as CD3+CD8+ (J) and then separated in differentiation subsets using CD45RA/CCR7 (K) or CD45RA/CD27 markers (L); CD38 and CD56 expression were evaluated on CD8+ T cells in M and N, respectively. Isotypic controls were employed to set the gates in F-I, K-N plots.
Fig. 2
Fig. 2. : total lymphocyte, CD3+ T cell, CD4+ T cell, CD8+ T cell, B cell and natural killer (NK) cell counts in American children and adolescents from Pediatric AIDS Clinical Trials Group P1009 (PACTG P1009) study and from Brazilian children and adolescents distributed in similar age strata. Data are presented in median values for each age stratum, with 95% confidence interval (CI) shown for the Brazilian cohort. Study data are considered statistically different if median values from PACTG P1009 study are not included in the 95% CI of the Brazilian cohort. m: months; y: years.
Fig. 3
Fig. 3. : CD4+ T and CD8+ T cells in American children and adolescents from Pediatric AIDS Clinical Trials Group P1009 (PACTG P1009) study and in Brazilian children and adolescents distributed in similar age strata. Cells are analysed according to percentage and absolute values of naïve subsets (CD45RA+) and percentage of CD38+ cells. Study data are considered statistically different if median values from PACTG P1009 study are not included in the 95% confidence interval of the Brazilian cohort. m: months; y: years.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

  • Ataxia-telangiectasia in Latin America: clinical features, immunodeficiency, and mortality in a multicenter study.
    Pereira RA, Dantas EO, Loekmanwidjaja J, Mazzucchelli JTL, Aranda CS, Serrano MEG, De La Cruz Córdoba EA, Bezrodnik L, Moreira I, Ferreira JFS, Dantas VM, Sales VSF, Fernandez CC, Vilela MMS, Motta IP, Franco JL, Arango JCO, Álvarez-Álvarez JA, Cardozo LRR, Orellana JC, Condino-Neto A, Kokron CM, Barros MT, Regairaz L, Cabanillas D, Suarez CLN, Rosario NA, Chong-Neto HJ, Takano OA, Nadaf MISV, Moraes LSL, Tavares FS, Rabelo F, Pino J, Calderon WC, Mendoza-Quispe D, Goudouris ES, Patiño V, Montenegro C, Souza MS, Branco AB, Forte WCN, Carvalho FAA, Segundo G, Cheik MFA, Roxo-Junior P, Peres M, Oliveira AM, Neto ACP, Ortega-López MC, Lozano A, Lozano NA, Nieto LH, Grumach AS, Costa DC, Antunes NMN, Nudelman V, Pereira CTM, Martinez MDM, Quiroz FJR, Cardona AA, Nuñez-Nuñez ME, Rodriguez JA, Cuellar CM, Vijoditz G, Bichuetti-Silva DC, Prando CCM, Amantéa SL, Costa-Carvalho BT. Pereira RA, et al. Immunol Res. 2024 Aug;72(4):864-873. doi: 10.1007/s12026-024-09494-5. Epub 2024 Jun 4. Immunol Res. 2024. PMID: 38834764
  • Evolution of CD4 T-Cell Count With Age in a Cohort of Young People Growing Up With Perinatally Acquired Human Immunodeficiency Virus.
    Castro H, Sabin C, Collins IJ, Okhai H, Schou Sandgaard K, Prime K, Foster C, Le Prevost M, Crichton S, Klein N, Judd A; Collaborative HIV Paediatric Study; UK Collaborative HIV Cohort Study. Castro H, et al. Clin Infect Dis. 2024 Mar 20;78(3):690-701. doi: 10.1093/cid/ciad626. Clin Infect Dis. 2024. PMID: 37820036 Free PMC article.
  • High Inborn Errors of Immunity Risk in Patients with Granuloma.
    Süleyman M, Serin O, Koçali B, Orhan D, Yalçın EE, Özçelik U, Ceyhan M, Kiper N, Tezcan İ, Dogru D, Çağdaş D. Süleyman M, et al. J Clin Immunol. 2022 Nov;42(8):1795-1809. doi: 10.1007/s10875-022-01342-1. Epub 2022 Aug 18. J Clin Immunol. 2022. PMID: 35980494
  • SCID and Other Inborn Errors of Immunity with Low TRECs - the Brazilian Experience.
    Barreiros LA, Sousa JL, Geier C, Leiss-Piller A, Kanegae MPP, França TT, Boisson B, Lima AM, Costa-Carvalho BT, Aranda CS, de Moraes-Pinto MI, Segundo GRS, Ferreira JFS, Tavares FS, Guimarães FATM, Toledo EC, da Matta Ain AC, Moreira IF, Soldatelli G, Grumach AS, de Barros Dorna M, Weber CW, Di Gesu RSW, Dantas VM, Fernandes FR, Torgerson TR, Ochs HD, Bustamante J, Walter JE, Condino-Neto A. Barreiros LA, et al. J Clin Immunol. 2022 Aug;42(6):1171-1192. doi: 10.1007/s10875-022-01275-9. Epub 2022 May 3. J Clin Immunol. 2022. PMID: 35503492
  • A Critical Review on the Standardization and Quality Assessment of Nonfunctional Laboratory Tests Frequently Used to Identify Inborn Errors of Immunity.
    Perazzio SF, Palmeira P, Moraes-Vasconcelos D, Rangel-Santos A, de Oliveira JB, Andrade LEC, Carneiro-Sampaio M. Perazzio SF, et al. Front Immunol. 2021 Nov 9;12:721289. doi: 10.3389/fimmu.2021.721289. eCollection 2021. Front Immunol. 2021. PMID: 34858394 Free PMC article. Review.
See all "Cited by" articles

References

    1. Abdelltef HM, Osman IM, Hilmi ZA, Altayeb OA. The reference ranges for lymphocytes subsets of healthy adults individuals by immunophenotyping. Aust J Basic Appl Sci. 2014;8:514–520.
    1. Berrington JE, Barge D, Fenton AC, Cant AJ, Spickett GP. Lymphocyte subsets in term and significantly preterm UK infants in the first year of life analysed by single platform flow cytometry. Clin Exp Immunol. 2005;140:289–292. - PMC - PubMed
    1. Calado RT, Garcia AB, Falcão RP. Age-related changes of immunophenotypically immature lymphocytes in normal human peripheral blood. Cytometry. 1999;38:133–137. - PubMed
    1. CDC - Centers for Disease Control and Prevention 1994 revised classification system for human immunodeficiency virus infection in children less than 13 years of age. MMWR Recomm Rep. 1994;43:1–10.
    1. Chini EN. CD38 as a regulator of cellular NAD: a novel potential pharmacological target for metabolic conditions. Curr Pharm Des. 2009;15:57–63. - PMC - PubMed

Publication types

MeSH terms

Substances