Dual enhancer activities of the cyclic-AMP responsive element with cell type and promoter specificity
- PMID: 2537960
- PMCID: PMC331819
- DOI: 10.1093/nar/17.4.1521
Dual enhancer activities of the cyclic-AMP responsive element with cell type and promoter specificity
Abstract
The role of the cyclic-AMP (cAMP) responsive element (CRE) in eukaryotic gene transcription was investigated in several cell lines transfected by constructs containing the chloramphenicol acetyltransferase (CAT) gene linked to the three different promoters, simian virus (SV) 40, human c-Ha-ras-1, or chicken beta-actin promoter, with or without CRE. CRE had inducible enhancer activity only when it was linked to the SV40 promoter and in a few cell lines such as PC12. CRE functioned as a constitutive enhancer with the human c-Ha-ras-1 promoter in all cell lines examined. CRE also had constitutive enhancer activity when it was linked to the chicken beta-actin promoter, but this activity was observed only in KB, HeLa, and A431 cells. The different types of enhancer activities of CRE depending on the cell and promoter may be caused by interaction with different trans-acting factors that were demonstrated by gel retardation analyses.
Similar articles
-
Enhancer and promoter element interactions dictate cyclic adenosine monophosphate mediated and cell-specific expression of the glycoprotein hormone alpha-gene.Mol Endocrinol. 1989 May;3(5):763-72. doi: 10.1210/mend-3-5-763. Mol Endocrinol. 1989. PMID: 2474127
-
Role of the cyclic adenosine 3',5'-monophosphate response element in efficient expression of the rat thyrotropin receptor promoter.Mol Endocrinol. 1992 Oct;6(10):1701-15. doi: 10.1210/mend.6.10.1333054. Mol Endocrinol. 1992. PMID: 1333054
-
Induction of chinook salmon growth hormone promoter activity by the adenosine 3',5'-monophosphate (cAMP)-dependent pathway involves two cAMP-response elements with the CGTCA motif and the pituitary-specific transcription factor Pit-1.Endocrinology. 1996 May;137(5):1775-84. doi: 10.1210/endo.137.5.8612514. Endocrinology. 1996. PMID: 8612514
-
Distinct adenosine 3',5'-monophosphate and phorbol ester-responsive signal transduction pathways converge at the level of transcriptional activation by the interactions of DNA-binding proteins.Mol Endocrinol. 1989 May;3(5):868-80. doi: 10.1210/mend-3-5-868. Mol Endocrinol. 1989. PMID: 2547158
-
Binding specificity of cyclic adenosine 3',5'-monophosphate-responsive element (CRE)-binding proteins and activating transcription factors to naturally occurring CRE sequence variants.Mol Endocrinol. 1991 Oct;5(10):1541-51. doi: 10.1210/mend-5-10-1541. Mol Endocrinol. 1991. PMID: 1837842
Cited by
-
Identification of cis- and trans-acting factors regulating the expression of rat salivary-specific RP4 gene.Gene Expr. 1992;2(4):365-77. Gene Expr. 1992. PMID: 1335317 Free PMC article.
-
Cyclic AMP response element-binding protein and the catalytic subunit of protein kinase A are present in F9 embryonal carcinoma cells but are unable to activate the somatostatin promoter.Mol Cell Biol. 1992 Mar;12(3):1096-106. doi: 10.1128/mcb.12.3.1096-1106.1992. Mol Cell Biol. 1992. PMID: 1347642 Free PMC article.
-
The cellular transcription factor CREB corresponds to activating transcription factor 47 (ATF-47) and forms complexes with a group of polypeptides related to ATF-43.Mol Cell Biol. 1990 Dec;10(12):6192-203. doi: 10.1128/mcb.10.12.6192-6203.1990. Mol Cell Biol. 1990. PMID: 2147221 Free PMC article.
-
Identification and functional characterisation of the cellular activating transcription factor 43 (ATF-43) protein.Nucleic Acids Res. 1991 Sep 11;19(17):4601-9. doi: 10.1093/nar/19.17.4601. Nucleic Acids Res. 1991. PMID: 1653949 Free PMC article.
-
Involvement of tyrosine kinase and protein kinase C in platelet-activating-factor-induced c-fos gene expression in A-431 cells.Biochem J. 1992 Sep 1;286 ( Pt 2)(Pt 2):527-33. doi: 10.1042/bj2860527. Biochem J. 1992. PMID: 1382409 Free PMC article.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials
Miscellaneous
