Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2015 Feb;42(2):329-36.
doi: 10.1007/s11033-014-3754-9. Epub 2014 Nov 6.

An infectious molecular clone in early infection with HIV-1 subtype CRF01_AE strains: construction and biological properties

Affiliations

An infectious molecular clone in early infection with HIV-1 subtype CRF01_AE strains: construction and biological properties

Hong-Wei Wang et al. Mol Biol Rep. 2015 Feb.

Retraction in

Abstract

Our aim was to construct infectious molecular clones of the CRF01_AE subtype in the primary infection phase of an acute HIV-1 infections in people screened from MSM populations, as well as continue preliminary research on this virus and its biological properties pertaining to deriving viruses. Walking sequencing was performed on a half-molecular clone with target fragment inserted. Western Blot was used to detect protein expression in HIV-1 infected 293T cells. Sequence analysis of HIV-1 genomic clones showed full-length HIV-1 genomic clones without frame shift mutation or termination codon. HIV-1 p24 antigens generated from 08-IMC were slightly greater than those from infectious molecular clones pNL4-3 3 and 93JP-NH1, but without statistical difference (all P > 0.05). The relative light units of 08-ISO was higher than those of 08-IMC, but no significant difference was observed (all P > 0.05). 08-IMC-driven virus was linked to lower replication kinetics. The replication levels of pNL4-3 and 08-ISO were significantly higher than the 08-IMC replication level but close to NH1 replication level (all P < 0.05). 08-IMC could infect the cells expressing CCR5 and be replicated in the CCR5-expressing cells with a positive percentage of 24.3 %, 08-ISO may use CCR5-using macrophage-tropic isolates as coreceptor, while pNL4-3 viruses with T cell tropisms utilize the CXCR4 co-receptor. Our study showed that the infectious molecular clones of viruses in the primary infection phase have a close relationship with the major prevalent CRF01_AE strains and have high homology with the viral RNA in plasma.

PubMed Disclaimer

Similar articles

Cited by

  • The Interplay of HIV and Autophagy in Early Infection.
    Cabrera-Rodríguez R, Pérez-Yanes S, Estévez-Herrera J, Márquez-Arce D, Cabrera C, Espert L, Blanco J, Valenzuela-Fernández A. Cabrera-Rodríguez R, et al. Front Microbiol. 2021 Apr 28;12:661446. doi: 10.3389/fmicb.2021.661446. eCollection 2021. Front Microbiol. 2021. PMID: 33995324 Free PMC article. Review.
  • Different Patterns of HIV-1 Replication in MACROPHAGES is Led by Co-Receptor Usage.
    Borrajo A, Ranazzi A, Pollicita M, Bellocchi MC, Salpini R, Mauro MV, Ceccherini-Silberstein F, Perno CF, Svicher V, Aquaro S. Borrajo A, et al. Medicina (Kaunas). 2019 Jun 21;55(6):297. doi: 10.3390/medicina55060297. Medicina (Kaunas). 2019. PMID: 31234437 Free PMC article.

References

    1. MMWR Surveill Summ. 2011 Oct 28;60(14):1-34 - PubMed
    1. Virology. 2013 Feb 5;436(1):33-48 - PubMed
    1. PLoS One. 2009 Aug 18;4(8):e6666 - PubMed
    1. PLoS Med. 2006 Nov;3(11):e443 - PubMed
    1. Drug Alcohol Depend. 2011 Jul 1;116(1-3):24-30 - PubMed

Publication types

LinkOut - more resources