Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1989 Mar;63(3):1095-100.
doi: 10.1128/JVI.63.3.1095-1100.1989.

Characterization of simian virus 40 receptor moieties on the surfaces of Vero C1008 cells

E T Clayson  1 , R W Compans
Affiliations

Characterization of simian virus 40 receptor moieties on the surfaces of Vero C1008 cells

E T Clayson et al. J Virol. 1989 Mar.

Abstract

The nature of the simian virus 40 (SV40) receptor on the surfaces of Vero C1008 cells was investigated by a virus binding assay. The optimum pH for SV40 binding to cell surfaces was found to be at 6.5; however, there was little difference in SV40 binding in the range between pH 4.5 and 7.3. The treatment of cell surfaces with several proteases or with an enzyme specific for O-linked carbohydrates significantly reduced virus binding, suggesting that the receptor for SV40 contains protein and O-linked carbohydrates. Treatment of cell monolayers with octyl glucoside removed virus-binding activity from cell surfaces. Recovery of virus-binding activity by octyl glucoside-treated cells took 2.5 h and was inhibited by cycloheximide or tunicamycin. Four polypeptides with molecular weights of 90,000, 58,000, 54,000, and 30,000 were immunoprecipitated from virus-protein complexes derived from octyl glucoside extract solutions and therefore may be components of the SV40 receptor. Competition experiments between SV40 and polyomavirus revealed that these two viruses do not share the same receptor on Vero C1008 cells.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. J Gen Virol. 1987 Sep;68 ( Pt 9):2477-80 - PubMed
    1. J Virol. 1987 Sep;61(9):2747-53 - PubMed
    1. Virus Res. 1988 Apr;10(1):41-51 - PubMed
    1. Mol Cell Biol. 1988 Aug;8(8):3391-6 - PubMed
    1. Proc Soc Exp Biol Med. 1962 Nov;111:367-72 - PubMed

Publication types

LinkOut - more resources