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. 2014 Oct;20(4):475-86.
doi: 10.1007/s12298-014-0246-2. Epub 2014 Jul 3.

Characterization of phytochemicals and evaluation of anti-cancer potential of Blumea eriantha DC

Mohini Gore  1 , N S Desai  1
Affiliations

Characterization of phytochemicals and evaluation of anti-cancer potential of Blumea eriantha DC

Mohini Gore et al. Physiol Mol Biol Plants. 2014 Oct.

Abstract

The aim of the present study was to evaluate the in vitro anti-cancer and anti-oxidant potential of methanol extract of Blumea eriantha as well as its phytochemical characterization. The in vitro cytotoxic and antiproliferative activities of B. eriantha methanolic extract of leaves were evaluated using MTT assay on HeLa and B16F10 cell lines, and wound scratch and colony formation assays on B16F10 cell lines. The expressions of p53 and Bcl-2 genes were also determined by reverse transcriptase-polymerase chain reaction to establish apoptosis. Isolation and identification of chemical constituents were carried out by various chromatographic and spectroscopic analytical techniques including HPTLC and LC-MS. The methanol extract of the leaves of B. eriantha showed potent in vitro antioxidant and anticancer properties. Moreover, the extract showed significant loss of wound healing, thus suggesting that it could prevent a possible metastasis. Hence, B. eriantha could be explored as a potential anticancer plant with antimetastatic properties.

Keywords: Apoptosis; Blumea eriantha; Cancer; Chromatography; Cytotoxicity; LC-MS.

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Figures

Fig. 1
Fig. 1
DPPH radical scavenging activity of leaf extract of B. eriantha in different solvent systems, data expressed in mean ± SD, n = 3
Fig. 2
Fig. 2
FRAP assay of leaf extract of B. eriantha in different solvent systems, data expressed in mean ± SD, n = 3
Fig. 3
Fig. 3
MTT assay on HeLa cell line showing cytotoxicity of the serially diluted methanolic leaf extracts of B. eriantha. % inhibition of cells increases with increasing concentration of the extract. IC50 value is 782.25 μg/ml
Fig. 4
Fig. 4
MTT assay on B16F10 cell line showing cytotoxicity of the serially diluted methanolic leaf extracts of B. eriantha. IC50 value is 841.84 μg/ml
Fig. 5
Fig. 5
MTT assay on B16F10 cells using methanolic leaf extracts of B. eriantha. a Control well with no cell death b well with extract concentration of 1 mg/ml showing a few crystals (viable cells) (c and d) wells with extract concentration of 2 mg/ml and 4 mg/ml respectively showing apoptotic cells
Fig. 6
Fig. 6
Wound scratch assay on B16F10 cells using methanolic leaf extracts of B. eriantha. a scratch wound at time of scratching, t = 0 b control plate 48 h after wounding, 34.41 % wound closure c test plate with 841.84 μl/ml (IC50 value) of extract 48 h after wounding, 1.5 % wound closure
Fig. 7
Fig. 7
Expression of housekeeping gene β actin, apoptotic gene p53 and antiapoptotic gene Bcl-2 in B16F10 cells treated with methanolic leaf extract of B. eriantha for 48 h. p53 level is upregulated and Bcl-2 is downregulated compared to negative control and doxorubicin. Representative data from three individual analyses have been shown here
Fig. 8
Fig. 8
HPTLC chromatogram of methanolic leaf extract of Blumea eriantha showing different peaks of phytoconstituents
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