Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1989 Jul;8(7):2023-8.
doi: 10.1002/j.1460-2075.1989.tb03610.x.

Leucine zipper structure of the protein CRE-BP1 binding to the cyclic AMP response element in brain

T Maekawa  1 , H Sakura, C Kanei-Ishii, T Sudo, T Yoshimura, J Fujisawa, M Yoshida, S Ishii
Affiliations

Leucine zipper structure of the protein CRE-BP1 binding to the cyclic AMP response element in brain

T Maekawa et al. EMBO J. 1989 Jul.

Abstract

By screening a lambda gt11 library with the multimerized sequence of the cAMP response element (CRE), we isolated human clones encoding the CRE binding protein, CRE-BP1, from a human brain cDNA library. CRE-BP1 expressed in Escherichia coli bound not only to the CRE element of the somatostatin and fibronectin genes, but also to the CRE element of the adenovirus E4 gene, suggesting that the protein was not distinguishable from the adenovirus transcription factor, ATF. The human CRE-BP1 clone encoded a 54.5 kd protein similar at its carboxy terminus to the leucine zipper motifs found in other enhancer binding proteins such as C/EBP and c-jun/AP-1. CRE-BP1 mRNA was expressed in all of the cells examined and was abundant in brain. The structure of CRE-BP1 and its recognition elements suggest that cellular response to extracellular stimuli is controlled by a family of transcription factors that bind to related cis-active elements and that contain several highly conserved domains.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 - PubMed
    1. Biochemistry. 1979 Nov 27;18(24):5294-9 - PubMed
    1. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 - PubMed
    1. Nature. 1984 Apr 19-25;308(5961):693-8 - PubMed
    1. Annu Rev Biochem. 1985;54:931-76 - PubMed