Role of bacteriophage Mu C protein in activation of the mom gene promoter
- PMID: 2522924
- PMCID: PMC209852
- DOI: 10.1128/jb.171.4.2019-2027.1989
Role of bacteriophage Mu C protein in activation of the mom gene promoter
Abstract
The phage Mu C gene product is a specific activator of Mu late gene transcription, including activation of the mom operon. Fusion of the C gene to the efficient translation initiation region of the Escherichia coli atpE gene allowed significant overproduction of C protein, which was subsequently purified and assayed for DNA binding by gel retardation and nuclease footprinting techniques. C protein binds to a site immediately upstream of the -35 region both of the mom promoter and the related phage D108 mod promoter. The location of the mom promoter has been determined by primer extension. Upstream deletions extending more than 3 base pairs into the C-binding site abolished activation of the mom promoter in vivo. In vitro binding of C was not significantly affected by DNA methylation. A second, C-dependent promoter was identified just downstream of the C coding region; comparison with the mom promoter revealed common structural elements.
Similar articles
-
Escherichia coli OxyR protein represses the unmethylated bacteriophage Mu mom operon without blocking binding of the transcriptional activator C.Nucleic Acids Res. 1996 Oct 15;24(20):4042-9. doi: 10.1093/nar/24.20.4042. Nucleic Acids Res. 1996. PMID: 8918810 Free PMC article.
-
Functionally distinct RNA polymerase binding sites in the phage Mu mom promoter region.Nucleic Acids Res. 1992 Jun 11;20(11):2777-84. doi: 10.1093/nar/20.11.2777. Nucleic Acids Res. 1992. PMID: 1535436 Free PMC article.
-
Bidirectional transcription in the mom promoter region of bacteriophage Mu.J Mol Biol. 1998 Dec 11;284(4):885-92. doi: 10.1006/jmbi.1998.2228. J Mol Biol. 1998. PMID: 9837712
-
Unusual transcriptional and translational regulation of the bacteriophage Mu mom operon.Pharmacol Ther. 1999 Dec;84(3):367-88. doi: 10.1016/s0163-7258(99)00042-x. Pharmacol Ther. 1999. PMID: 10665835 Review.
-
The mom gene of bacteriophage Mu.Curr Top Microbiol Immunol. 1984;108:29-47. doi: 10.1007/978-3-642-69370-0_4. Curr Top Microbiol Immunol. 1984. PMID: 6232116 Review. No abstract available.
Cited by
-
Bacteriophage Mu late promoters: four late transcripts initiate near a conserved sequence.J Bacteriol. 1989 Apr;171(4):2003-18. doi: 10.1128/jb.171.4.2003-2018.1989. J Bacteriol. 1989. PMID: 2522923 Free PMC article.
-
Identification of a positive regulator of the Mu middle operon.J Bacteriol. 1990 Dec;172(12):6641-50. doi: 10.1128/jb.172.12.6641-6650.1990. J Bacteriol. 1990. PMID: 2147678 Free PMC article.
-
Regulation of bacteriophage Mu transposition.Genetica. 1994;93(1-3):27-39. doi: 10.1007/BF01435237. Genetica. 1994. PMID: 7813916 Review.
-
Mutational analysis of a C-dependent late promoter of bacteriophage Mu.Genetics. 1993 Nov;135(3):619-29. doi: 10.1093/genetics/135.3.619. Genetics. 1993. PMID: 8293968 Free PMC article.
-
Activation of bacteriophage Mu mom transcription by C protein does not require specific interaction with the carboxyl-terminal region of the alpha or sigma 70 subunit of Escherichia coli RNA polymerase.J Bacteriol. 1998 Jun;180(12):3257-9. doi: 10.1128/JB.180.12.3257-3259.1998. J Bacteriol. 1998. PMID: 9620983 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
