Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1989 Nov;27(11):2492-6.
doi: 10.1128/jcm.27.11.2492-2496.1989.

Detection of Mycoplasma pneumoniae by using the polymerase chain reaction

C Bernet  1 , M Garret, B de Barbeyrac, C Bebear, J Bonnet
Affiliations
Comparative Study

Detection of Mycoplasma pneumoniae by using the polymerase chain reaction

C Bernet et al. J Clin Microbiol. 1989 Nov.

Abstract

The polymerase chain reaction (PCR) technique was used to detect Mycoplasma pneumoniae. A specific DNA sequence for M. pneumoniae was selected from a genomic library, and two oligonucleotides were chosen in this sequence to give an amplified fragment of 144 base pairs. We show that DNA from different M. pneumoniae strains can be detected by PCR, with DNA from other Mycoplasma species giving negative results. Analysis of biological samples (throat swabs) obtained from hamsters that were experimentally infected with M. pneumoniae showed that PCR was more sensitive and reliable than conventional culture techniques for the detection of M. pneumoniae. Initial experiments on artificially seeded human bronchoalveolar lavages showed that PCR can be used to detect 10(2) to 10(3) organisms.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Tex Rep Biol Med. 1965 Jun;23:Suppl 1:285+ - PubMed
    1. Res Microbiol. 1989 Jan;140(1):7-16 - PubMed
    1. Science. 1977 Mar 4;195(4281):892-4 - PubMed
    1. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 - PubMed
    1. Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 - PubMed

Publication types

LinkOut - more resources