New soluble-formazan assay for HIV-1 cytopathic effects: application to high-flux screening of synthetic and natural products for AIDS-antiviral activity
- PMID: 2495366
- DOI: 10.1093/jnci/81.8.577
New soluble-formazan assay for HIV-1 cytopathic effects: application to high-flux screening of synthetic and natural products for AIDS-antiviral activity
Erratum in
- J Natl Cancer Inst 1989 Jun 21;81(12):963
Abstract
We have developed an effective and optimally safe microculture method for rapid and convenient assay of the in vitro cytopathic effects of human immunodeficiency virus (HIV-1) on human lymphoblastoid or other suitable host cells. The assay procedure is applicable to the evaluation of drug effects on in vitro infections induced directly in cultured host cells by cell-free HIV-1 or by coculture with H9 cells chronically infected with HIV-1. The assay uses a newly developed tetrazolium reagent that is metabolically reduced by viable cells to yield a soluble, colored formazan product measurable by conventional colorimetric techniques. This simple microassay minimizes the number of plate manipulations typically required with other assay methods and, coupled with computerized data collection and analysis, facilitates large-scale screening of agents for potential antiviral activity. To support and enhance the discovery of new anti-HIV-1 agents, the National Cancer Institute is offering investigators worldwide the opportunity to submit new candidate agents for anti-HIV-1 screening with this method.
Similar articles
-
The inhibitory effect of the crude extract from a seaweed of Dygenea simplex C. Agardh on the in vitro cytopathic activity of HIV-1 and it's antigen production.Chem Pharm Bull (Tokyo). 1995 Sep;43(9):1580-4. doi: 10.1248/cpb.43.1580. Chem Pharm Bull (Tokyo). 1995. PMID: 7586085
-
Effects of tubeimoside-1 on HIV core protein p24 and cytopathogenesis in vitro.Zhongguo Yao Li Xue Bao. 1994 Mar;15(2):103-6. Zhongguo Yao Li Xue Bao. 1994. PMID: 8010099
-
AIDS-antiviral sulfolipids from cyanobacteria (blue-green algae).J Natl Cancer Inst. 1989 Aug 16;81(16):1254-8. doi: 10.1093/jnci/81.16.1254. J Natl Cancer Inst. 1989. PMID: 2502635
-
Rapid screening of antiretroviral combinations.J Acquir Immune Defic Syndr Hum Retrovirol. 1995;10 Suppl 1:S24-7. J Acquir Immune Defic Syndr Hum Retrovirol. 1995. PMID: 8595504 Review.
-
Evaluation of new antiviral agents: I. In vitro perspectives.Antiviral Res. 1989 Jun-Jul;11(5-6):217-32. doi: 10.1016/0166-3542(89)90032-6. Antiviral Res. 1989. PMID: 2679375 Review.
Cited by
-
The HBV drug entecavir - effects on HIV-1 replication and resistance.N Engl J Med. 2007 Jun 21;356(25):2614-21. doi: 10.1056/NEJMoa067710. N Engl J Med. 2007. PMID: 17582071 Free PMC article.
-
New approaches for quantitating the inhibition of HIV-1 replication by antiviral drugs in vitro and in vivo.Curr Opin Infect Dis. 2009 Dec;22(6):574-82. doi: 10.1097/QCO.0b013e328332c54d. Curr Opin Infect Dis. 2009. PMID: 19841584 Free PMC article. Review.
-
Antiviral Effects of ABMA against Herpes Simplex Virus Type 2 In Vitro and In Vivo.Viruses. 2018 Mar 9;10(3):119. doi: 10.3390/v10030119. Viruses. 2018. PMID: 29522484 Free PMC article.
-
Effect of Foeniculum Vulgare Aqueous and Alcoholic Seed Extract against Zoonotic Cutaneous Leishmaniasis.Ethiop J Health Sci. 2021 Mar;31(2):401-408. doi: 10.4314/ejhs.v31i2.23. Ethiop J Health Sci. 2021. PMID: 34158792 Free PMC article.
-
Physicochemical Properties and Antiherpetic Activity of κ-Carrageenan Complex with Chitosan.Mar Drugs. 2023 Apr 13;21(4):238. doi: 10.3390/md21040238. Mar Drugs. 2023. PMID: 37103377 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
