Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2014 Aug;8(7-8):488-505.
doi: 10.1002/prca.201400014. Epub 2014 Jul 14.

Quantitative proteomics in cardiovascular research: global and targeted strategies

Xiaomeng Shen  1 , Rebeccah Young, John M Canty, Jun Qu
Affiliations
Review

Quantitative proteomics in cardiovascular research: global and targeted strategies

Xiaomeng Shen et al. Proteomics Clin Appl. 2014 Aug.

Abstract

Extensive technical advances in the past decade have substantially expanded quantitative proteomics in cardiovascular research. This has great promise for elucidating the mechanisms of cardiovascular diseases and the discovery of cardiac biomarkers used for diagnosis and treatment evaluation. Global and targeted proteomics are the two major avenues of quantitative proteomics. While global approaches enable unbiased discovery of altered proteins via relative quantification at the proteome level, targeted techniques provide higher sensitivity and accuracy, and are capable of multiplexed absolute quantification in numerous clinical/biological samples. While promising, technical challenges need to be overcome to enable full utilization of these techniques in cardiovascular medicine. Here, we discuss recent advances in quantitative proteomics and summarize applications in cardiovascular research with an emphasis on biomarker discovery and elucidating molecular mechanisms of disease. We propose the integration of global and targeted strategies as a high-throughput pipeline for cardiovascular proteomics. Targeted approaches enable rapid, extensive validation of biomarker candidates discovered by global proteomics. These approaches provide a promising alternative to immunoassays and other low-throughput means currently used for limited validation.

Keywords: Biomarker; Cardiovascular diseases; LC-MS; Mechanism study; Targeted quantification.

PubMed Disclaimer

Figures

Figure. 1
Figure. 1. A generic workflow of global quantitative proteomics using a bottom-up (shotgun) approach
Figure. 2
Figure. 2. Label-free quantification strategies
(A) Illustration of the work flow of the ion current-based relative quantification; (B) comparison of the reproducibility of quantitative features by ion current-based vs. spectral count methods for the repetitive analysis of a tissue sample (reproduced from Ref[34]).
Figure. 3
Figure. 3
A high-throughput method development pipeline for sensitive, accurate and reproducible LC/SRM-MS quantification of target proteins in complex matrices, based on an on-the-fly orthogonal array optimization and experimental evaluation. Details can be found in Ref. [136, 137].
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Yusuf S, Reddy S, Ounpuu S, Anand S. Global burden of cardiovascular diseases: Part II: variations in cardiovascular disease by specific ethnic groups and geographic regions and prevention strategies. Circulation. 2001;104:2855–2864. - PubMed
    1. Cui Z, Dewey S, Gomes AV. Cardioproteomics: advancing the discovery of signaling mechanisms involved in cardiovascular diseases. Am J Cardiovasc Dis. 2011;1:274–292. - PMC - PubMed
    1. Arab S, Gramolini AO, Ping P, Kislinger T, et al. Cardiovascular proteomics: tools to develop novel biomarkers and potential applications. Journal of the American College of Cardiology. 2006;48:1733–1741. - PubMed
    1. Hoofnagle AN, Wener MH. The fundamental flaws of immunoassays and potential solutions using tandem mass spectrometry. J Immunol Methods. 2009;347:3–11. - PMC - PubMed
    1. Schnabel RB, Baccarelli A, Lin H, Ellinor PT, Benjamin EJ. Next steps in cardiovascular disease genomic research--sequencing, epigenetics, and transcriptomics. Clinical Chemistry. 2012;58:113–126. - PMC - PubMed

Publication types

LinkOut - more resources