The application of transcription activator-like effector nucleases for genome editing in C. elegans
- PMID: 24780522
- DOI: 10.1016/j.ymeth.2014.04.013
The application of transcription activator-like effector nucleases for genome editing in C. elegans
Abstract
The nematode Caenorhabditis elegans has been a powerful model system for biomedical research in the past decades, however, the efficient genetic tools are still demanding for gene knockout, knock-in or conditional gene mutations. Transcription activator-like effector nucleases (TALENs) that comprise a sequence-specific DNA-binding domain fused to a FokI nuclease domain facilitate the targeted genome editing in various cell types or organisms. Here we summarize the recent progresses and protocols using TALENs in C. elegans that generate gene mutations and knock-ins in the germ line and the conditional gene knockout in somatic tissues.
Keywords: Caenorhabditis elegans; Conditional gene knockout; Mutation; Transcription activator-like effector nucleases (TALENs).
Copyright © 2014 Elsevier Inc. All rights reserved.
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