Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2014 May;15(5):321-34.
doi: 10.1038/nrg3686. Epub 2014 Apr 2.

A guide to genome engineering with programmable nucleases

Hyongbum Kim  1 , Jin-Soo Kim  2
Affiliations
Review

A guide to genome engineering with programmable nucleases

Hyongbum Kim et al. Nat Rev Genet. 2014 May.

Abstract

Programmable nucleases - including zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and RNA-guided engineered nucleases (RGENs) derived from the bacterial clustered regularly interspaced short palindromic repeat (CRISPR)-Cas (CRISPR-associated) system - enable targeted genetic modifications in cultured cells, as well as in whole animals and plants. The value of these enzymes in research, medicine and biotechnology arises from their ability to induce site-specific DNA cleavage in the genome, the repair (through endogenous mechanisms) of which allows high-precision genome editing. However, these nucleases differ in several respects, including their composition, targetable sites, specificities and mutation signatures, among other characteristics. Knowledge of nuclease-specific features, as well as of their pros and cons, is essential for researchers to choose the most appropriate tool for a range of applications.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Cell. 2014 Feb 13;156(4):836-43 - PubMed
    1. Nat Biotechnol. 2013 Dec;31(12):1137-42 - PubMed
    1. Nat Methods. 2014 Feb;11(2):122-3 - PubMed
    1. Nature. 2005 Jun 2;435(7042):646-51 - PubMed
    1. Science. 2009 Dec 11;326(5959):1509-12 - PubMed

Publication types

LinkOut - more resources