Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1988 Feb;102(2):397-407.
doi: 10.1242/dev.102.2.397.

Isolation and expression of a new mouse homeobox gene

P T Sharpe  1 , J R Miller, E P Evans, M D Burtenshaw, S J Gaunt
Affiliations

Isolation and expression of a new mouse homeobox gene

P T Sharpe et al. Development. 1988 Feb.

Abstract

A homeobox-containing clone has been isolated from an adult mouse kidney cDNA library and shown by DNA sequence analysis to be a new isolate, Hox-6.1. A genomic clone containing Hox-6.1 has been isolated and found to contain another putative homeobox sequence (Hox-6.2), within 7 kb of Hox-6.1. In situ hybridization of mouse metaphase chromosomes shows this Hox-6 locus to be located on chromosome 14 (14E2). Hox-6.1 has been studied in detail and the predicted protein sequence of the homeobox is 100% homologous to the Xenopus Xeb1 (formally AC1) homeobox and the human c8 homeobox (Carrasco et al. 1984; Boncinelli et al. 1985; Simeone et al. 1987). Southern blotting shows that the DNA sequence encoding Hox-6.1 is single copy. Expression of Hox-6.1 has been studied in adult tissues and embryos by RNase protection assays, Northern blotting analysis and in situ hybridization. RNase protection assays show that Hox-6.1 transcripts are present in embryos between days 9 1/2 and 13 1/2 of gestation and in extraembryonic tissues at day 9 1/2. Adult expression is detectable in kidney and testis but not in liver, spleen and brain. One major transcript is detectable on Northern blots of kidney and day-13 1/2 embryo RNA. In kidney, this transcript is 2.7 kb whereas in embryos the major transcript is smaller at 1.9 kb, a much fainter band being visible at 2.7 kb. Localized expression of Hox-6.1 is observed in the spinal cord and prevertebral column of day-12 1/2 embryos, and in the posterior mesoderm and ectoderm of day-8 1/4 embryos. An anterior boundary of expression is located just behind the hindbrain whereas the boundary in the mesoderm is located at the level of the 7th prevertebra.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles