Effects of fixation and processing on the immunohistochemical visualization of type-I, -III and -IV collagen in paraffin-embedded liver tissue
- PMID: 2449408
- DOI: 10.1007/BF00490172
Effects of fixation and processing on the immunohistochemical visualization of type-I, -III and -IV collagen in paraffin-embedded liver tissue
Abstract
We compared the effects of different fixatives and enzymatic-digestion procedures on the immunohistochemical visualization of type-I, -III and -IV collagen in paraffin-embedded normal human liver sections. None of the fixatives tested allowed the staining of these antigens without prior enzymatic digestion. The best results i.e. strong staining intensity and well-defined localization, were obtained when liver tissue was fixed in Bouin's fluid or in other solutions containing picric acid. Several other fixatives, including Carnoy's fluid, Lillie's AAF, 10% neutral formalin and 96% ethanol, gave unsatisfactory results. Pepsin was ineffective for unmasking type-I and -III collagen antigens, and was only partially effective for visualizing the type-IV collagen antigen. The best results were obtained when material fixed in Bouin's fluid was embedded in paraffin and digested with trypsin. Using this procedure, the results were comparable to those obtained in unfixed frozen sections with respect to the staining intensity, specificity and non-specific staining.
Similar articles
-
The influence of fixation and tissue preparation on the immunohistochemical demonstration of fibronectin in human tissue.Histochemistry. 1981;72(2):291-9. doi: 10.1007/BF00517142. Histochemistry. 1981. PMID: 6168612
-
The effect of fixation and trypsinization on the immunohistochemical demonstration of intracellular immunoglobulin in paraffin embedded material.Acta Pathol Microbiol Scand A. 1980 Nov;88(6):369-76. doi: 10.1111/j.1699-0463.1980.tb02508.x. Acta Pathol Microbiol Scand A. 1980. PMID: 6163317
-
Immunohistochemical detection of VHS virus in paraffin-embedded specimens of rainbow trout (Oncorhynchus mykiss): the influence of primary antibody, fixative, and antigen unmasking on method sensitivity.Vet Pathol. 1997 May;34(3):253-61. doi: 10.1177/030098589703400316. Vet Pathol. 1997. PMID: 9163887
-
The importance of tissue fixation for light microscopic immunohistochemical localization of peroxisomal proteins: the superiority of Carnoy's fixative over Baker's formalin and Bouin's solution.Histochem Cell Biol. 1995 Mar;103(3):187-95. doi: 10.1007/BF01454023. Histochem Cell Biol. 1995. PMID: 7553132
-
Effects of fixation and enzymatic digestion on the immunohistochemical demonstration of laminin and fibronectin in paraffin embedded tissue.J Clin Pathol. 1984 Jun;37(6):639-44. doi: 10.1136/jcp.37.6.639. J Clin Pathol. 1984. PMID: 6427296 Free PMC article.
Cited by
-
Picrosirius Red Staining: Revisiting Its Application to the Qualitative and Quantitative Assessment of Collagen Type I and Type III in Tendon.J Histochem Cytochem. 2021 Oct;69(10):633-643. doi: 10.1369/00221554211046777. Epub 2021 Sep 22. J Histochem Cytochem. 2021. PMID: 34549650 Free PMC article.
-
Chronic hibernating myocardium: interstitial changes.Mol Cell Biochem. 1995 Jun 7-21;147(1-2):35-42. doi: 10.1007/BF00944781. Mol Cell Biochem. 1995. PMID: 7494552
-
On the Nature of Tintinnid Loricae (Ciliophora: Spirotricha: Tintinnina): a Histochemical, Enzymatic, EDX, and High-resolution TEM Study.Acta Protozool. 2012 Jun 8;51(1):1-19. Acta Protozool. 2012. PMID: 22988335 Free PMC article.