Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1987 Dec;61(12):3726-32.
doi: 10.1128/JVI.61.12.3726-3732.1987.

Activation of the human immunodeficiency virus by herpes simplex virus type 1

J M Ostrove  1 , J Leonard, K E Weck, A B Rabson, H E Gendelman
Affiliations

Activation of the human immunodeficiency virus by herpes simplex virus type 1

J M Ostrove et al. J Virol. 1987 Dec.

Abstract

Herpes simplex virus type 1 (HSV-1) and some of its immediate-early genes stimulate expression of the human immunodeficiency virus (HIV) long terminal repeat (LTR) sequences and the replication of HIV itself. To demonstrate this, the HIV LTR was linked to the indicator gene chloramphenicol acetyltransferase (CAT) and transfected into Vero cells with or without the trans-activating gene (tat) of HIV. Infection of these cells with HSV-1 strain KOS or temperature-sensitive mutant tsB21 or tsE6 resulted in a large increase in CAT activity in the absence of tat and further augmentation in the presence of tat. This stimulation was seen at both their permissive (34 degrees C) and nonpermissive (39 degrees C) temperatures, implying either that HSV-1 infection or immediate-early gene expression is all that is required. In cotransfection assays in Vero cells, cloned HSV-1 immediate-early genes ICP0 and ICP4 stimulated CAT activity in the presence of tat, while ICP27 had no effect. On the other hand, in SW480 cells, ICP4 and, to a lesser extent, ICP0 genes caused stimulation of CAT activity in the absence of tat. Deletion mutants within the HIV LTR showed that the target for HSV stimulation is distinct from the tat-responsive area and maps near the SP1 binding sites. In Hela cells, ICP0 or ICP4 stimulated the replication of a cotransfected clone of HIV, as shown by an increase in reverse transcriptase activity in the culture supernatant.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Virology. 1973 Apr;52(2):456-67 - PubMed
    1. Proc Natl Acad Sci U S A. 1987 Jan;84(1):71-5 - PubMed
    1. Anal Biochem. 1976 May 7;72:248-54 - PubMed
    1. J Immunol. 1978 Jan;120(1):130-6 - PubMed
    1. Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 - PubMed

Substances

LinkOut - more resources