Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1988 Jan;19(1-2):7-22.
doi: 10.1007/BF00114566.

Using bacteria to analyze sequences involved in chloroplast gene expression

A A Gatenby  1 , S J Rothstein, D Bradley
Affiliations

Using bacteria to analyze sequences involved in chloroplast gene expression

A A Gatenby et al. Photosynth Res. 1988 Jan.

Abstract

The expression of higher plant chloroplast genes in prokaryotic cells has been used to examine organelle sequences involved in promoter recognition by RNA polymerase, and protein translocation through membranes. The similarity in sequence structure between Escherichia coli promoters and the maize chloroplast atpB promoter has been investigated using deletion and single base pair substitution mutants. The atpB mutants were mainly isolated by a selection system in E. coli, and then used as templates for the analysis of transcription using chloroplast RNA polymerase. It was found that both the bacterial and chloroplast RNA polymerases behaved in a similar fashion with the wild-type and mutant promoters, indicating that the sequences involved in promoter recognition share a considerable degree of homology. Signal peptide recognition of pea cytochrome f has also been examined in E. coli. This signal peptide, which is probably responsible for insertion of the protein into the thylakoid membrane, is efficiently recognized in E. coli leading to the inner membrane insertion of petA::lacZ fusion proteins. This process requires the bacterial SecA protein and points to a general similarity in the mechanisms of protein translocation within chloroplasts and bacteria.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. EMBO J. 1984 Aug;3(8):1697-704 - PubMed
    1. Annu Rev Biochem. 1986;55:879-912 - PubMed
    1. J Biol Chem. 1986 Nov 5;261(31):14804-10 - PubMed
    1. EMBO J. 1985 Dec 30;4(13B):3641-8 - PubMed
    1. Mol Cell Biol. 1985 Oct;5(10):2733-45 - PubMed