Imaging dynamic redox processes with genetically encoded probes
- PMID: 24406687
- DOI: 10.1016/j.yjmcc.2013.12.023
Imaging dynamic redox processes with genetically encoded probes
Abstract
Redox signalling plays an important role in many aspects of physiology, including that of the cardiovascular system. Perturbed redox regulation has been associated with numerous pathological conditions; nevertheless, the causal relationships between redox changes and pathology often remain unclear. Redox signalling involves the production of specific redox species at specific times in specific locations. However, until recently, the study of these processes has been impeded by a lack of appropriate tools and methodologies that afford the necessary redox species specificity and spatiotemporal resolution. Recently developed genetically encoded fluorescent redox probes now allow dynamic real-time measurements, of defined redox species, with subcellular compartment resolution, in intact living cells. Here we discuss the available genetically encoded redox probes in terms of their sensitivity and specificity and highlight where uncertainties or controversies currently exist. Furthermore, we outline major goals for future probe development and describe how progress in imaging methodologies will improve our ability to employ genetically encoded redox probes in a wide range of situations. This article is part of a special issue entitled "Redox Signalling in the Cardiovascular System."
Keywords: Genetically encoded redox probes; Hydrogen peroxide; Superoxide; cpYFP; roGFP.
Copyright © 2014 Elsevier Ltd. All rights reserved.
Similar articles
-
Genetically encoded fluorescent redox sensors.Biochim Biophys Acta. 2014 Feb;1840(2):745-56. doi: 10.1016/j.bbagen.2013.05.030. Epub 2013 May 29. Biochim Biophys Acta. 2014. PMID: 23726987 Review.
-
Measuring E(GSH) and H2O2 with roGFP2-based redox probes.Free Radic Biol Med. 2011 Dec 1;51(11):1943-51. doi: 10.1016/j.freeradbiomed.2011.08.035. Epub 2011 Sep 10. Free Radic Biol Med. 2011. PMID: 21964034
-
Genetically encoded reactive oxygen species (ROS) and redox indicators.Biotechnol J. 2014 Feb;9(2):282-93. doi: 10.1002/biot.201300199. Biotechnol J. 2014. PMID: 24497389 Review.
-
Genetically encoded redox sensors.Methods Enzymol. 2014;542:263-87. doi: 10.1016/B978-0-12-416618-9.00014-5. Methods Enzymol. 2014. PMID: 24862271 Review.
-
Mechanisms and Applications of Redox-Sensitive Green Fluorescent Protein-Based Hydrogen Peroxide Probes.Antioxid Redox Signal. 2018 Aug 20;29(6):552-568. doi: 10.1089/ars.2017.7449. Epub 2018 Jan 10. Antioxid Redox Signal. 2018. PMID: 29160083 Review.
Cited by
-
Visualization of the Redox Status of Cytosolic Glutathione Using the Organelle- and Cytoskeleton-Targeted Redox Sensors.Antioxidants (Basel). 2020 Feb 3;9(2):129. doi: 10.3390/antiox9020129. Antioxidants (Basel). 2020. PMID: 32028573 Free PMC article.
-
Real-time monitoring of basal H2O2 levels with peroxiredoxin-based probes.Nat Chem Biol. 2016 Jun;12(6):437-43. doi: 10.1038/nchembio.2067. Epub 2016 Apr 18. Nat Chem Biol. 2016. PMID: 27089028
-
The role of thiols in antioxidant systems.Free Radic Biol Med. 2019 Aug 20;140:14-27. doi: 10.1016/j.freeradbiomed.2019.05.035. Epub 2019 Jun 13. Free Radic Biol Med. 2019. PMID: 31201851 Free PMC article. Review.
-
Interpreting Heterogeneity in Response of Cells Expressing a Fluorescent Hydrogen Peroxide Biosensor.Biophys J. 2015 Nov 17;109(10):2148-58. doi: 10.1016/j.bpj.2015.08.053. Biophys J. 2015. PMID: 26588573 Free PMC article.
-
Measuring intracellular concentration of hydrogen peroxide with the use of genetically encoded H2O2 biosensor HyPer.Redox Biol. 2019 Jun;24:101200. doi: 10.1016/j.redox.2019.101200. Epub 2019 Apr 16. Redox Biol. 2019. PMID: 31030065 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
