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. 2013:2013:186041.
doi: 10.1155/2013/186041. Epub 2013 Nov 14.

Circulating C19MC microRNAs in preeclampsia, gestational hypertension, and fetal growth restriction

Ilona Hromadnikova  1 , Katerina Kotlabova  1 , Marketa Ondrackova  1 , Andrea Kestlerova  2 , Veronika Novotna  2 , Lucie Hympanova  3 , Jindrich Doucha  4 , Ladislav Krofta  2
Affiliations

Circulating C19MC microRNAs in preeclampsia, gestational hypertension, and fetal growth restriction

Ilona Hromadnikova et al. Mediators Inflamm. 2013.

Abstract

The objective of the study was to identify the profile of circulating C19MC microRNAs (miR-516-5p, miR-517*, miR-518b, miR-520a*, miR-520h, miR-525, and miR-526a) in patients with established preeclampsia (n = 63), fetal growth restriction (n = 27), and gestational hypertension (n = 23). We examined the correlation between plasmatic concentrations and expression levels of microRNAs and the severity of the disease with respect to clinical signs, requirements for the delivery, and Doppler ultrasound parameters. Using absolute and relative quantification approaches, increased extracellular C19MC microRNA levels (miR-516-5p, P = 0.037, P = 0.009; miR-517*, P = 0.033, P = 0.043; miR-520a*, P = 0.001, P = 0.009; miR-525, P = 0.026, P = 0.01; miR-526a, P = 0.03, P = 0.035) were detected in patients with preeclampsia. The association analysis pointed to no relationship between C19MC microRNA plasmatic concentrations and expression profile and identified risk factors for a poorer perinatal outcome. However, the dependence between the levels of plasmatic C19MC microRNAs and the pulsatility index in the middle cerebral artery and the values of cerebroplacental ratio was demonstrated. The study brought the interesting finding that the upregulation of miR-516-5p, miR-517*, miR-520a*, miR-525, and miR-526a is a characteristic phenomenon of established preeclampsia.

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Figures

Figure 1
Figure 1
Upregulation of circulating C19MC microRNAs in pregnancies with preeclampsia. Absolute ((a), (b), (c), (d), and (e)) and relative ((f), (g), (h), (i), and (j)) quantification data were expressed as box plots of individual microRNAs in cohorts of normal and complicated pregnancies using Statistica software. The upper and lower limits of the boxes represent the 75th and 25th percentiles, respectively. The upper and lower whiskers represent the maximum and minimum values that are no more than 1.5 times the span of the interquartile range (range of the values between the 25th and the 75th percentiles). The median is indicated by the line in each box. Outliers are indicated by circles and extremes by asterisks.
Figure 2
Figure 2
The association between the plasmatic concentration (a) and expression levels (b) of C19MC microRNAs and Doppler ultrasonography parameters. ρ: Spearman's correlation coefficient; P: level of significance.
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References

    1. Redman CWG, Sargent IL. Preeclampsia and the systemic inflammatory response. Seminars in Nephrology. 2004;24(6):565–570. - PubMed
    1. Nelson DM. Apoptotic changes occur in syncytiotrophoblast of human placental villi where fibrin type fibrinoid is deposited at discontinuities in the villous trophoblast. Placenta. 1996;17(7):387–391. - PubMed
    1. Oudejans CBM, Tjoa ML, Westerman BA, Mulders MAM, Van Wijk IJ, Van Vugt JMG. Circulating trophoblast in maternal blood. Prenatal Diagnosis. 2003;23(2):111–116. - PubMed
    1. Huppertz B, Kingdom JCP. Apoptosis in the trophoblast—role of apoptosis in placental morphogenesis. Journal of the Society for Gynecologic Investigation. 2004;11(6):353–362. - PubMed
    1. Orozco AF, Bischoff FZ, Horne C, Popek E, Simpson JL, Lewis DE. Hypoxia-induced membrane-bound apoptotic DNA particles: potential mechanism of fetal DNA in maternal plasma. Annals of the New York Academy of Sciences. 2006;1075:57–62. - PubMed

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