Skip to main page content
U.S. flag

An official website of the United States government

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1986 Aug 26;14(16):6507-23.
doi: 10.1093/nar/14.16.6507.

Chicken histone genes retain nuclear matrix association throughout the cell cycle

S Dalton, H B Younghusband, J R Wells
Free PMC article

Chicken histone genes retain nuclear matrix association throughout the cell cycle

S Dalton et al. Nucleic Acids Res. .
Free PMC article

Abstract

The association between histone genes and the nuclear matrix (NM) during periods of high (S-phase) and low (non-S-phase) transcriptional activity has been investigated with synchronized cells from a chicken erythroid cell line (abbreviated ts34). By DNase I and restriction enzyme analysis, these studies reveal that both core and linker histone genes (represented by H2A and H1 genes respectively) are attached to the NM independent of their transcriptional activity during the cell-cycle. The tissue-specific histone gene H5, expressed constitutively, is nuclear matrix (NM)-associated in ts34 cells but is found in the supernatant (S/N) fractions of a non-erythroid T-cell line. Furthermore, we show that DNA sequences necessary for NM-attachment of the H5 gene lie within a 780 base pair region spanning part of the coding and 5' non-translated region. Of the three non-histone genes investigated, beta-actin sequences are expressed and are NM-attached, feather keratin genes are not expressed and predominate in the S/N, and beta-globin genes although not expressed in the ts34 cell line used were found in the NM fraction. In this case the association may be fortuitous or may reflect an early event prior to transcription of globin genes in differentiating erythroid cells. These results generally support the notion that actively transcribed genes are NM-attached, but that attachment per se is not synonymous with transcription.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Cell. 1976 Nov;9(3):393-407 - PubMed
    1. Science. 1976 Sep 3;193(4256):848-56 - PubMed
    1. J Cell Sci. 1976 Nov;22(2):287-302 - PubMed
    1. Cell. 1977 Nov;12(3):805-16 - PubMed
    1. Cell. 1977 Nov;12(3):817-28 - PubMed

Publication types