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. 1985 Dec;5(12):3640-3.
doi: 10.1128/mcb.5.12.3640-3643.1985.

Isolation of antibodies for phosphotyrosine by immunization with a v-abl oncogene-encoded protein

J Y Wang

Isolation of antibodies for phosphotyrosine by immunization with a v-abl oncogene-encoded protein

J Y Wang. Mol Cell Biol. 1985 Dec.

Abstract

Immunization of rabbits with a tyrosine-phosphorylated v-abl protein resulted in the production of antibodies for the v-abl protein and for phosphotyrosine. The antiphosphotyrosine antibodies could be purified by affinity chromatography with O-phosphotyramine coupled to Sepharose. These antibodies detected a variety of tyrosine-phosphorylated proteins, including receptors for peptide growth factors. The usefulness of these antibodies was demonstrated by the detection of previously unidentified tyrosine-phosphorylated proteins in v-src-, v-abl-, and v-erbB-transformed cell lines.

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