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Comment
. 2014 May;134(5):1479-1481.
doi: 10.1038/jid.2013.483. Epub 2013 Nov 12.

Merkel cell polyomavirus-positive Merkel cell carcinoma requires viral small T-antigen for cell proliferation

Masahiro Shuda  1 , Yuan Chang #  1 , Patrick S Moore #  1
Affiliations
Comment

Merkel cell polyomavirus-positive Merkel cell carcinoma requires viral small T-antigen for cell proliferation

Masahiro Shuda et al. J Invest Dermatol. 2014 May.
No abstract available

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Figures

Figure 1
Figure 1
Detection of Merkel cell polyomavirus (MCV) small T (sT) antigen expression by multiple MCV T antigen antibodies. MCV-positive MCC cells (MKL-1, MKL-2, MS-1 and WaGa) and MCV-negative UISO cells transfected with MCV genomic T antigen gene or empty vector as positive and negative controls, were immunoblotted with multiple MCV T antigen antibodies. αTubulin was used as a loading control. Both large T (LT, closed arrows) and small T (sT, open arrows) were detected by CM8E6 and 2T2, sT by CM5E1, and LT by CM2B4. Asterisks indicate non-specific bands. Peptide sequences used for monoclonal antibody production and shRNA targeting sites are shown in the bottom diagram of T antigen transcripts with a DnaJ (green box), an Rb-binding (black box) as well as large T stabilization (LSD, yellow box (Kwun et al., 2013)) domains.
Figure 2
Figure 2
Merkel cell polyomavirus (MCV) small T (sT) antigen knockdown inhibits MCV-positive Merkel cell carcinoma (MCC) cell proliferation. (a) MCV-positive MCC cell lines, MKL-1 and MKL-2, were transduced with pLKO.1-based lentiviral shRNAs targeting both LT and sT (panT.MS) or sT alone (sT1.MS, sT1.RH, and sT2.RH) as described (Houben et al., 2010). Both LT (closed arrows) and sT (open arrows) proteins are detected by 2T2. (b) Expression of LT and sT was quantitated by LI-COR IR immunoblotting system using αTubulin for normalization. Relative sT expression to sh ctrl is shown. (c) shRNA-transduced MCV-positive (MKL-1 and MKL-2) cells and MCV-negative (UISO) cells were subjected to Wst-1 cell proliferation assay. Error bars indicate standard deviation.
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Comment in

  • Response to Shuda et al.
    Angermeyer S, Hesbacher S, Becker JC, Schrama D, Houben R. Angermeyer S, et al. J Invest Dermatol. 2014 May;134(5):1481-1482. doi: 10.1038/jid.2013.486. Epub 2013 Nov 12. J Invest Dermatol. 2014. PMID: 24217012 No abstract available.

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References

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