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. 2013;3(3):105-9.
doi: 10.5681/bi.2013.028. Epub 2013 Sep 17.

Intrinsic bio-signature of gene delivery nanocarriers may impair gene therapy goals

Jaleh Barar  1 , Yadollah Omidi
Affiliations

Intrinsic bio-signature of gene delivery nanocarriers may impair gene therapy goals

Jaleh Barar et al. Bioimpacts. 2013.

Abstract

Non-viral lipid/polymeric vectors have widely been used as nanocarriers (NCs) for gene delivery. They possess large surface area to volume ratio and are able to interact with biomolecules through functional moieties, resulting in inadvertent biological impacts, in particular at genomic level. Thus, their genomic bio-signature needs to be investigated prior to use in vivo. Using high-throughput microarray and qPCR gene expression profiling techniques, we have reported the genomic impacts of lipid/polymeric NCs. Given the fact that the ultimate objectives of gene therapy may inevitably be impaired by nonspecific intrinsic genomic impacts of these NCs, here, we highlight their nonspecific genomic bio-signature. We envision that better understanding on the genotoxicity of gene delivery NCs, as guiding premise, will help us to develop much safer NCs and also to accelerate their translation into clinical use and to provide pivotal information on safety liabilities early in discovery and developments process prior to its inevitable consequences in vivo.

Keywords: Gene Delivery; Gene Therapy; Genotoxicity; Microarray; Nanotoxicology; Toxicogenomics.

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References

    1. Rubanyi GM. The future of human gene therapy. Mol Aspects Med . 2001;22:113–42. - PubMed
    1. Barar J, Omidi Y. Translational Approaches toward Cancer Gene Therapy: Hurdles and Hopes. BioImpacts . 2012;2:127–43. - PMC - PubMed
    1. Hughes MD, Hussain M, Nawaz Q, Sayyed P, Akhtar S. The cellular delivery of antisense oligonucleotides and ribozymes. Drug Discov Today . 2001;6:303–15. - PubMed
    1. Somia N, Verma IM. Gene therapy: trials and tribulations. Nat Rev Genet . 2000;1:91–9. - PubMed
    1. Kerr D. Clinical development of gene therapy for colorectal cancer. Nat Rev Cancer . 2003;3:615–22. - PubMed

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