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. 2013 Oct 14;8(10):e77116.
doi: 10.1371/journal.pone.0077116. eCollection 2013.

Identification and characterization of eleven novel human gamma-papillomavirus isolates from healthy skin, found at low frequency in a normal population

Jingjing Li  1 , YaQi Pan, QiuJu Deng, Hong Cai, Yang Ke
Affiliations

Identification and characterization of eleven novel human gamma-papillomavirus isolates from healthy skin, found at low frequency in a normal population

Jingjing Li et al. PLoS One. .

Erratum in

  • PLoS One. 2013;8(12). doi:10.1371/annotation/974c14e0-9cd1-4600-8259-abdc7927a318
  • PLoS One. 2014;9(1). doi:10.1371/annotation/fa94362e-5639-42d9-b17b-f89c51c4e9bb

Abstract

Eleven novel human papillomavirus (HPV) types were isolated and characterized from healthy individuals in China. HPV163 belongs to the γ-1 species, HPV 164 and HPV 168 fit in the γ-8 species, HPV 165 and KC5 belongs to the γ-12 species, HPV 168 is closely allied with the γ-4 species, HPV 169 is closely related to the γ-11 species, and HPV 170 is related to the γ-12 species. In addition, HPV 161, HPV 162, and HPV 166 may form a new HPV species of the γ-PV genus. The prevalence of these HPV types in the normal population is low.

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Conflict of interest statement

Competing Interests: The authors have declared that no competing interests exist.

Figures

Figure 1
Figure 1. Systematic positions of novel HPVs.
Eleven novel HPVs (HPV 161-170, KC5) were nested within the Gammapapillomavirus Genus. The evolutionary history was inferred by using the Bayesian and ML methods. A Bayesian consensus tree was based on the whole length of the L1 ORFs. Numbers on branches are bootstrap support values to the clusters to their right (upper number: ML criterion, values, 50 are not shown) and Bayesian posterior probabilities (lower number, values, 0.90 are not shown). The tree is rooted with selected Mu and Nu species. The analysis involved 68 nucleotide sequences. All positions containing gaps and missing data were eliminated. There were a total of 1263 positions in the final dataset. Bar, 0.2 nucleotide substitutions per site. *: Particular types show incongruent systematic positions as inferred from E1-E2 gene phylogenies (Supplementary file).
Figure 2
Figure 2. Strategy of amplification of the intact novel HPV genome.
(A) Reverse PCR primers were design according to the known FA fragment nested in the L1 ORF to amplify the almost intact HPV genome, but lack of a small part of the genome; (B) Reverse PCR primers were designed according to the sequence obtained from (A), and primers were located inside the LCR region.
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Grants and funding

This work was supported by the grants from the 973 project (201202014), the National Natural Sciences Foundation of China (30930102), the Beijing Natural Science Foundation (7100001), and the Specialized Research Fund for the Doctoral Program of Higher Education of China (Grant No. 20110001110037). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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