Autographa californica M nuclear polyhedrosis virus: microtubules and replication
- PMID: 2408230
- DOI: 10.1016/0042-6822(90)90211-9
Autographa californica M nuclear polyhedrosis virus: microtubules and replication
Abstract
Progressive reorganization and depolymerization of microtubules corresponded with virus-induced rounding of Autographa californica M nuclear polyhedrosis virus (AcMNPV)-infected Spodoptera frugiperda IPLB-Sf-21 cells, suggesting that microtubules were instrumental in maintaining the normal shape of these cells. Depolymerization of all cortical and most of the paranuclear microtubules with colchicine also resulted in cell rounding, confirming this hypothesis. Studies with aphidicolin and cycloheximide indicated the virus-induced effects on the microtubules were mediated by both early and late viral gene products. Microtubules in cells infected with a p10 deletion mutant depolymerized microtubules in a manner similar to those in wild-type virus-infected cells, indicating p10 was not responsible for virus-induced changes in the microtubules. Nevertheless, evidence for the association of p10 and microtubules was obtained by fluorescence microscopy and immunoelectron microscopy. Colchicine depolymerization of microtubules before and throughout infection did not interfere with virus replication, but treatment of cells with taxol, a microtubule-stabilizing agent, both delayed and depressed virus replication. The taxol-induced effect was relieved by the addition of colchicine. These results suggested that AcMNPV-induced depolymerization of microtubules may be a necessary event in, rather than a tangential effect of, virus replication. Attempts to monitor the effects of virus infection on intermediate filaments were unsuccessful due to the lack of cross-reactivity between antibodies to intermediate filament proteins and IPLB-Sf-21 cells, indicating these proteins are not highly conserved in lepidopteran insect cells.
Similar articles
-
Functional studies on the p10 gene of Autographa californica nuclear polyhedrosis virus using a recombinant expressing a p10-beta-galactosidase fusion gene.J Gen Virol. 1988 Apr;69 ( Pt 4):765-76. doi: 10.1099/0022-1317-69-4-765. J Gen Virol. 1988. PMID: 3128641
-
Construction and analysis of an Autographa californica nuclear polyhedrosis virus mutant lacking the polyhedral envelope.Virology. 1989 Nov;173(1):98-108. doi: 10.1016/0042-6822(89)90225-0. Virology. 1989. PMID: 2683364
-
Association of Autographa californica nuclear polyhedrosis virus (AcMNPV) with the nuclear matrix.Virology. 1988 Nov;167(1):233-41. doi: 10.1016/0042-6822(88)90073-6. Virology. 1988. PMID: 3055664
-
[Nuclear polyhedrosis viruses of insects].Uirusu. 1984 Dec;34(2):73-88. doi: 10.2222/jsv.34.73. Uirusu. 1984. PMID: 6397907 Review. Japanese. No abstract available.
-
An overview of the structure and replication of baculoviruses.Curr Top Microbiol Immunol. 1986;131:1-19. doi: 10.1007/978-3-642-71589-1_1. Curr Top Microbiol Immunol. 1986. PMID: 3545691 Review. No abstract available.
Cited by
-
Replication patterns and cytopathology of cells infected with baculoviruses.Cytotechnology. 1996 Jan;20(1-3):95-110. doi: 10.1007/BF00350391. Cytotechnology. 1996. PMID: 22358475
-
The baculoviruses occlusion-derived virus: virion structure and function.Adv Virus Res. 2007;69:99-165. doi: 10.1016/S0065-3527(06)69003-9. Adv Virus Res. 2007. PMID: 17222693 Free PMC article. Review.
-
Baculovirus Actin Rearrangement-Inducing Factor 1 Can Remodel the Mammalian Actin Cytoskeleton.Microbiol Spectr. 2023 Feb 13;11(2):e0518922. doi: 10.1128/spectrum.05189-22. Online ahead of print. Microbiol Spectr. 2023. PMID: 36779726 Free PMC article.
-
Ezrin NH2-terminal domain inhibits the cell extension activity of the COOH-terminal domain.J Cell Biol. 1995 Mar;128(6):1081-93. doi: 10.1083/jcb.128.6.1081. J Cell Biol. 1995. PMID: 7896873 Free PMC article.
-
Identification of the early actin-rearrangement-inducing factor gene, arif-1, from Autographa californica multicapsid nuclear polyhedrosis virus.J Virol. 1997 Oct;71(10):7933-41. doi: 10.1128/JVI.71.10.7933-7941.1997. J Virol. 1997. PMID: 9311884 Free PMC article.