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Comparative Study
. 2013 Sep;51(9):2977-85.
doi: 10.1128/JCM.00911-13. Epub 2013 Jul 3.

Comparison of the FilmArray RP, Verigene RV+, and Prodesse ProFLU+/FAST+ multiplex platforms for detection of influenza viruses in clinical samples from the 2011-2012 influenza season in Belgium

Liesbeth Van Wesenbeeck  1 , Hanne Meeuws, Andrea Van Immerseel, Gabriela Ispas, Kristiane Schmidt, Lieselot Houspie, Marc Van Ranst, Lieven Stuyver
Affiliations
Comparative Study

Comparison of the FilmArray RP, Verigene RV+, and Prodesse ProFLU+/FAST+ multiplex platforms for detection of influenza viruses in clinical samples from the 2011-2012 influenza season in Belgium

Liesbeth Van Wesenbeeck et al. J Clin Microbiol. 2013 Sep.

Abstract

Respiratory tract infections (RTIs) are caused by a plethora of viral and bacterial pathogens. In particular, lower RTIs are a leading cause of hospitalization and mortality. Timely detection of the infecting respiratory pathogens is crucial to optimize treatment and care. In this study, three U.S. Food and Drug Administration-approved molecular multiplex platforms (Prodesse ProFLU+/FAST+, FilmArray RP, and Verigene RV+) were evaluated for influenza virus detection in 171 clinical samples collected during the Belgian 2011-2012 influenza season. Sampling was done using mid-turbinate flocked swabs, and the collected samples were stored in universal transport medium. The amount of viral RNA present in the swab samples ranged between 3.07 and 8.82 log10 copies/ml. Sixty samples were concordant influenza A virus positive, and 8 samples were found to be concordant influenza B virus positive. Other respiratory viruses that were detected included human rhinovirus/enterovirus, respiratory syncytial virus, parainfluenza virus type 1, human metapneumovirus, and coronavirus NL63. Twenty-five samples yielded discordant results across the various assays which required further characterization by sequencing. The FilmArray RP and Prodesse ProFLU+/FAST+ assays were convenient to perform with regard to sensitivity, ease of use, and low percentages of invalid results. Although the limit of sensitivity is of utmost importance, many other factors should be taken into account in selecting the most convenient molecular diagnostic assay for the detection of respiratory pathogens in clinical samples.

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Figures

Fig 1
Fig 1
Viral load range for influenza A viruses in 92 clinical samples. A total of 79 samples were negative for the influenza A viral load assay.
See this image and copyright information in PMC

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References

    1. Hammond SP, Gagne LS, Stock SR, Marty FM, Gelman RS, Marasco WA, Poritz MA, Baden LR. 2012. Respiratory virus detection in immunocompromised patients with FilmArray respiratory panel compared to conventional methods. J. Clin. Microbiol. 50:3216–3221 - PMC - PubMed
    1. Loeffelholz MJ, Pong DL, Pyles RB, Xiong Y, Miller AL, Bufton KK, Chonmaitree T. 2011. Comparison of the FilmArray respiratory panel and Prodesse real-time PCR assays for detection of respiratory pathogens. J. Clin. Microbiol. 49:4083–4088 - PMC - PubMed
    1. Jennings LC, Anderson TP, Werno AM, Beynon KA, Murdoch DR. 2004. Viral etiology of acute respiratory tract infections in children presenting to hospital: role of polymerase chain reaction and demonstration of multiple infections. Pediatr. Infect. Dis. J. 23:1003–1007 - PubMed
    1. Byington CL, Castillo H, Gerber K, Daly JA, Brimley LA, Adams S, Christenson JC, Pavia AT. 2002. The effect of rapid respiratory viral diagnostic testing on antibiotic use in a children's hospital. Arch. Pediatr. Adolesc. Med. 156:1230–1234 - PubMed
    1. Cho CH, Woo MK, Kim JY, Cheong S, Lee CK, An SA, Lim CS, Kim WJ. 2013. Evaluation of five rapid diagnostic kits for influenza A/B virus. J. Virol. Methods 187:51–56 - PubMed