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. 2013 Jul;54 Suppl 1(Suppl 1):i13-22.
doi: 10.1093/jrr/rrs135.

Influence of chronic hypoxia and radiation quality on cell survival

Ning-Yi Ma  1 , Walter Tinganelli, Andreas Maier, Marco Durante, Wilma Kraft-Weyrather
Affiliations

Influence of chronic hypoxia and radiation quality on cell survival

Ning-Yi Ma et al. J Radiat Res. 2013 Jul.

Abstract

To investigate the influence of chronic hypoxia and anoxia on cell survival after low- and high-LET radiation, CHO-K1 cells were kept for 24 h under chronic hypoxia (94.5% N2; 5% CO2; 0.5% O2) or chronic anoxia (95% N2; 5% CO2). Irradiation was performed using 250 kVp X-rays or carbon ions with a dose average LET of 100 keV/μm either directly under the chronic oxygenation states, or at different time points after reoxygenation. Moreover, the cell cycle distribution for cells irradiated under different chronic oxic states was measured over 24 h during reoxygenation. The measurements showed a fairly uniform cell cycle distribution under chronic hypoxia, similar to normoxic conditions. Chronic anoxia induced a block in G1 and a strong reduction of S-phase cells. A distribution similar to normoxic conditions was reached after 12 h of reoxygenation. CHO cells had a similar survival under both acute and chronic hypoxia. In contrast, survival after irradiation under chronic anoxia was slightly reduced compared to that under acute anoxia. We conclude that, in hamster cells, chronic anoxia is less effective than acute anoxia in inducing radioresistance for both X-rays and carbon ions, whereas in hypoxia, acute and chronic exposures have a similar impact on cell killing.

Keywords: anoxia; carbon ions; cell cycle distribution; hypoxia; radiosensitivity.

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Figures

Fig. 1.
Fig. 1.
Survival of CHO-K1 cells after X-ray irradiation under different states of oxygenation. Data for acute states are from (W. Tinganelli et al., submitted for publication) (simultaneous experiments).
Fig. 2.
Fig. 2.
Survival of CHO-K1 cells after carbon ion irradiation under different states of oxygenation. Data for acute states are from (W. Tinganelli et al., submitted for publication) (simultaneous experiments).
Fig. 3.
Fig. 3.
Survival of CHO-K1 cells after irradiation under normoxia and at different times of reoxygenation from chronic anoxia or hypoxia. Data for acute states are from (W. Tinganelli et al., submitted for publication).
Fig. 4.
Fig. 4.
Cell cycle distribution in CHO-K1 cells under acute and chronic anoxic and hypoxic conditions.
Fig. 5.
Fig. 5.
Cell cycle distribution in CHO-K1 cells (A) during reoxygenation after chronic anoxia and hypoxia (closed symbols) compared to normoxic conditions (open symbols). (B) under reoxygenation after chronic anoxia and hypoxia. Time-points correspond to the survival curves in Fig. 3.
Fig. 6.
Fig. 6.
Cell cycle distribution after X-ray exposure under normoxia (open symbols) or chronic anoxia (closed symbols). In the last column to the right, data are shown for cells exposed to 6 Gy (normoxic) or 11.6 Gy (chronic anoxia), corresponding to the same survival level.
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