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. 2013 Jan;10(1):23-31.
Epub 2013 Jan 31.

Expression levels of th-2 and th-17 characteristic genes in healthy tissue versus periodontitis

Parichehr Behfarnia  1 , Reza Birang, Seyed Sajad Pishva, Mazdak Ghanjalikhani Hakemi, Mitra Mohammadi Khorasani
Affiliations

Expression levels of th-2 and th-17 characteristic genes in healthy tissue versus periodontitis

Parichehr Behfarnia et al. J Dent (Tehran). 2013 Jan.

Abstract

Objective: The T helper 17 (Th17) cells have been suggested to have osteoclast activating effects while T helper 2 (Th2) cells are considered to have an osteoprotective role in periodontitis. This study was to compare the markers of Th17 cells (RORC2 and IL-17 genes) with that of Th2 cells (IL-4 and GATA-3 genes) between healthy and periodontitis tissues.

Materials and methods: The samples were obtained from patients with periodontitis and healthy tissues. The mRNA expression levels of IL-17, RORC2, IL-4 and GATA-3 were measured in both groups using quantitative RT-PCR. The results were compared using SPSS 11.0. Correlation coefficient was analyzed by Spearman's rho test. Mann-Whitney was used to measure the difference between IL-17 and IL-4 as well as RORC2 and GATA-3.

Results: In periodontal lesions, the expression levels of all markers were significantly higher than the healthy tissue (p≤ 0.001). The results showed a significant increase in the number of markers of Th17 (RORC2 and IL-17 genes) compared to markers of Th2 (GATA-3, IL-4) in patients with periodontitis compared to controls (p≤0.002). A positive correlation between IL-17 and RORC2 (p≤0.05) and between IL-4 and GATA-3 (p≤0.001) was found.

Conclusion: The results show that expression levels of IL-4, GATA-3, IL-17 and RORC2 increase significantly in periodontal lesions compared with the controls. In periodontal lesions, IL-17 levels are significantly greater than IL-4, which plays a protective role against alveolar bone loss.

Keywords: GATA3 factors; IL-17F protein; Periodontal Disease; TH17 cell; TH2 cell.

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Figures

Fig 1
Fig 1
Gene expressions in periodontal lesions and healthy tissue
Fig 2
Fig 2
Correlation between gene expression of IL-17 and RORC2 in the periodontitis affected tissue (p≤0.001)
Fig 3
Fig 3
Correlation between gene expression of IL-4 and GATA-3 in the periodontitis affected tissue (p≤0.001)
See this image and copyright information in PMC

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