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Review

Hematopoietic differentiation

Kyung-Dal Choi  1 , Maxim Vodyanik  1 , Igor I Slukvin  1
In: StemBook [Internet]. Cambridge (MA): Harvard Stem Cell Institute; 2008.
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Review

Hematopoietic differentiation

Kyung-Dal Choi et al.
Free Books & Documents

Excerpt

A protocol for hematopoietic differentiation of human pluripotent stem cells (hPSCs) and generation of mature myeloid cells from hPSCs through expansion and differentiation of hPSC-derived lin−CD34+CD43+CD45+ multipotent progenitors. The protocol comprises three major steps: (i) induction of hematopoietic differentiation by coculture of hPSCs with OP9 bone marrow stromal cells; (ii) short-term expansion of multipotent myeloid progenitors with a high dose of granulocyte-macrophage colony-stimulating factor; and (iii) directed differentiation of myeloid progenitors into neutrophils, eosinophils, dendritic cells, Langerhans cells, macrophages and osteoclasts. The generation of multipotent hematopoietic progenitors from hPSCs requires 9 d of culture and an additional 2 d to expand myeloid progenitors. Differentiation of myeloid progenitors into mature myeloid cells requires an additional 5–19 d of culture with cytokines, depending on the cell type.

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References

    1. Choi K.-D, Vodyanik M, Slukvin I. I. Nature Protocols 6 296–313. 10.1038/nprot.2010.184, Published online 17 February 2011 2011 - DOI - PMC - PubMed

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