Fetuin-A mRNA expression is elevated in NASH compared with NAFL patients
- PMID: 23627434
- DOI: 10.1042/CS20120542
Fetuin-A mRNA expression is elevated in NASH compared with NAFL patients
Abstract
Fetuin-A is a pro-inflammatory protein expressed by hepatocytes. Its course in morbidly obese patients with NAFLD (non-alcoholic fatty liver disease) following weight loss by BAS (bariatric surgery) has not been fully elucidated yet. In the present study, we prospectively examined the effects of weight loss on various metabolic factors at 4 weeks and 6 months after surgery. Blood and liver tissues were retrieved from 108 morbidly obese NAFLD patients before/during BAS, and 50 of these individuals met the criteria for NASH (non-alcoholic steatohepatitis). Fetuin-A expression was measured by qPCR (quantitative real-time PCR), Western blotting and immunohistochemistry. Hepatocyte apoptosis was quantified via M30 (caspase-cleaved cytokeratin-18 fragments). Plasma concentrations of adiponectin and fetuin-A were determined by ELISA. Serum-derived parameters were additionally taken at 4 weeks and 6 months post-operatively. In addition, primary human hepatocytes were treated with NEFA (non-esterified fatty acid) to investigate changes in fetuin-A. BMI (body mass index) decreased significantly from 53.0±1.1 to 36.4±1.9 kg/m2 in the NAFL group and from 53.3±1.1 to 37.6±1.2 kg/m2 in the NASH group (P<0.0001) at 6 months post-surgery. This was associated with diminishing M30 and M65 (total cytokeratin-18) levels over 6 months after surgery. Adiponectin levels increased continuously in NASH patients, whereas NAFL patients plateaued at 4 weeks post-operatively. Hepatic fetuin-A mRNA and protein expression was elevated before surgery-induced weight loss. However, plasma concentrations of fetuin-A increased signficantly in NASH patients 4 weeks post-operatively. Treatment of hepatocytes with NEFA led to up-regulation of fetuin-A expression. BAS probably has a beneficial effect on NAFLD, as indicated by reduced hepatocyte apoptosis and improved adipokine profiles. In addition, fetuin-A expression is more prominent in NASH.
Similar articles
-
Plasma visfatin levels and gene expression in morbidly obese women with associated fatty liver disease.Clin Biochem. 2013 Feb;46(3):202-8. doi: 10.1016/j.clinbiochem.2012.11.006. Epub 2012 Nov 19. Clin Biochem. 2013. PMID: 23174488
-
Effects of weight loss induced by bariatric surgery on hepatic adipocytokine expression.J Hepatol. 2009 Oct;51(4):765-77. doi: 10.1016/j.jhep.2009.06.016. Epub 2009 Jul 5. J Hepatol. 2009. PMID: 19664840
-
Noninvasive clinical model for the diagnosis of nonalcoholic steatohepatitis in overweight and morbidly obese patients undergoing bariatric surgery.Chirurgia (Bucur). 2012 Nov-Dec;107(6):772-9. Chirurgia (Bucur). 2012. PMID: 23294957
-
Serum total adiponectin in nonalcoholic fatty liver disease: a systematic review and meta-analysis.Metabolism. 2011 Mar;60(3):313-26. doi: 10.1016/j.metabol.2010.09.003. Epub 2010 Oct 30. Metabolism. 2011. PMID: 21040935 Review.
-
Molecular and pathobiological involvement of fetuin-A in the pathogenesis of NAFLD.Inflammopharmacology. 2021 Aug;29(4):1061-1074. doi: 10.1007/s10787-021-00837-4. Epub 2021 Jun 29. Inflammopharmacology. 2021. PMID: 34185201 Review.
Cited by
-
The effects of HAP and macrophage cells to the expression of inflammatory factors and apoptosis in HK-2 cells of vitro co-cultured system.Urolithiasis. 2018 Oct;46(5):429-443. doi: 10.1007/s00240-017-1032-8. Epub 2017 Dec 13. Urolithiasis. 2018. PMID: 29236151 Free PMC article.
-
Systematic Review and Meta-analysis of Circulating Fetuin-A Levels in Nonalcoholic Fatty Liver Disease.J Clin Transl Hepatol. 2021 Feb 28;9(1):3-14. doi: 10.14218/JCTH.2020.00081. Epub 2020 Dec 9. J Clin Transl Hepatol. 2021. PMID: 33604250 Free PMC article.
-
Organ-organ communication: The liver's perspective.Theranostics. 2021 Jan 16;11(7):3317-3330. doi: 10.7150/thno.55795. eCollection 2021. Theranostics. 2021. PMID: 33537089 Free PMC article. Review.
-
The Wide and Complex Field of NAFLD Biomarker Research: Trends.ISRN Hepatol. 2014 Apr 28;2014:846923. doi: 10.1155/2014/846923. eCollection 2014. ISRN Hepatol. 2014. PMID: 27335843 Free PMC article. Review.
-
Pathophysiological Implication of Fetuin-A Glycoprotein in the Development of Metabolic Disorders: A Concise Review.J Clin Med. 2019 Nov 21;8(12):2033. doi: 10.3390/jcm8122033. J Clin Med. 2019. PMID: 31766373 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous