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. 2010 Mar;24(2):145-52.
doi: 10.1016/S1674-8301(10)60023-1.

Inhibition of TGF-β1 by eNOS gene transfer provides cardiac protection after myocardial infarction

Wei Qin  1 , Xin Chen, Peisheng Liu
Affiliations

Inhibition of TGF-β1 by eNOS gene transfer provides cardiac protection after myocardial infarction

Wei Qin et al. J Biomed Res. 2010 Mar.

Abstract

Objective: Endothelial nitric oxide synthase (eNOS) and nitric oxide (NO) have been implicated in protection against myocardial ischemia injury. This study was designed to explore a new method of therapy for myocardial injury by eNOS gene transfection.

Methods: A rat model of myocardial infarction (MI) was established by left anterior descending (LAD) coronary artery ligation. eNOS gene in an adenovirus vector was delivered locally into the rat heart and hemodynamic parameters were examined after 3 weeks, Matrix metalloproteinase-2 and 9 (MMP-2, MMP-9) mRNA were measured by reverse transcription polymerase chain reaction (RT-PCR), and the protein levels of eNOS, caspase-3, and transforming grouth factor β1 (TGF-β1) were determined by western blot assay.

Results: eNOS gene transfer significantly reduced cardiomyocyte apoptosis and improved cardiac function. In addition, eNOS significantly reduced the mRNA levels of MMP-2 and MMP-9. In the eNOS gene transfected group, the activation of caspase-3 and TGF-β1 were decreased. However, the protection was reversed by administration of the NOS inhibitor, N(ω)-nitro-l-arginine methyl ester (L-NAME).

Conclusion: These results demonstrate that the eNOS provides cardiac protection after myocardial infarction injury through inhibition of cardiac apoptosis and collagen deposition, and suppression of TGF-β1.

Keywords: cardiac function; cardiomyocyte apoptosis; collagen deposition; endothelial nitric oxide synthase gene; myocardial infarction; transforming growth factor-β1.

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Figures

Fig. 1
Fig. 1. The survival rate of each group at different times.
Fig. 2
Fig. 2. Identification of eNOS formation in the rat left ventricle at 3 weeks after MI. A: western blot analysis showing eNOS expression. B: Quantitative analysis of eNOS / Actin, *P < 0.05 means Ad.eNOS vs other MI groups.
Fig. 3
Fig. 3. RT-PCR showing the levels of MMP-2 (A), MMP-9 (B), and Actin (C) mRNA in each group, D: Quantitative analysis of relative intensity of MMP-2 and MMP-9, *P < 0.05 means Ad.eNOS vs other MI groups.
Fig. 4
Fig. 4. Expression of Caspase-3 (A, B) and TGF-β1 (C, D) in the rat left ventricle at 3 weeks after MI. A: western blot analysis showing Caspase-3 level. B: Quantitative analysis of Caspase-3 / Actin, *P < 0.05 means Ad.eNOS vs other MI groups. C: western blot analysis showing TGF-β1 level. D: Quantitative analysis of TGF-β1 / Actin, *P < 0.05 means Ad.eNOS vs other MI groups.
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